Recent biotechnological interventions for developing improved penicillin G acylases

被引：6

作者：

Rajendhran, J ^{[1
]}

Gunasekaran, P ^{[1
]}

机构：

[1] Madurai Kamaraj Univ, Sch Biol Sci, Ctr Excellence Genom Sci, Dept Microbial Technol, Madurai 625021, Tamil Nadu, India

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2004年 / 97卷 / 01期

关键词：

penicillin acylase; beta-lactam antibiotics; post-translational processing; enzyme stabilization; protein engineering; directed evolution;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Penicillin G acylase (PAC; EC 3.5.1.11) is the key enzyme used in the industrial production of beta-lactam antibiotics. This enzyme hydrolyzes the side chain of penicillin G and related P-lactam antibiotics releasing 6-amino penicillanic acid (6-APA), which is the building block in the manufacture of semisynthetic penicillins. PAC from Escherichia coli strain ATCC 11105, Bacillus megaterium strain ATCC 14945 and mutants of these two strains is currently used in industry. Genes encoding for PAC from various bacterial sources have been cloned and overexpressed with significant improvements in transcription, translation and post-translational processing. Recent developments in enzyme engineering have shown that PAC can be modified to gain conformational stability and desired functionality. This review provides an overview of recent advances in the production, stabilization and application of PAC, highlighting the recent biotechnological approaches for the improved catalysis of PAC.

引用

页码：1 / 13

页数：13

共 113 条

[11] EFFECTS OF SITE-DIRECTED MUTATIONS ON PROCESSING AND ACTIVITIES OF PENICILLIN-G ACYLASE FROM ESCHERICHIA-COLI ATCC-11105 [J].

CHOI, KS ;

KIM, JA ;

KANG, HS .

JOURNAL OF BACTERIOLOGY, 1992, 174 (19) :6270-6276

[12] Effect of SecB chaperone on production of periplasmic penicillin acylase in Escherichia coli [J].

Chou, CF ;

Tseng, JH ;

Kuo, BY ;

Lai, KM ;

Lin, MI ;

Lin, HK .

BIOTECHNOLOGY PROGRESS, 1999, 15 (03) :439-445

[13]

Chou CP, 1999, BIOTECHNOL BIOENG, V65, P219

[14]

Chou CP, 1999, BIOTECHNOL BIOENG, V63, P263, DOI 10.1002/(SICI)1097-0290(19990505)63:3<263::AID-BIT2>3.0.CO

[15]

2-T

[16] Genetic strategies to enhance penicillin acylase production in Escherichia coli [J].

Chou, CP ;

Lin, WJ ;

Kuo, BY ;

Yu, CC .

ENZYME AND MICROBIAL TECHNOLOGY, 2000, 27 (10) :766-773

[17] HYDROLYSIS OF PENICILLINS AND RELATED COMPOUNDS BY CELL-BOUND PENICILLIN ACYLASE OF ESCHERICHIA COLI [J].

COLE, M .

BIOCHEMICAL JOURNAL, 1969, 115 (04) :733-&

[18] DNA shuffling of a family of genes from diverse species accelerates directed evolution [J].

Crameri, A ;

Raillard, SA ;

Bermudez, E ;

Stemmer, WPC .

NATURE, 1998, 391 (6664) :288-291

[19] Expression of penicillin G acylase from the cloned pac gene of Escherichia coli ATCC11105 -: Effects of pacR and temperature [J].

Dai, MH ;

Zhu, YM ;

Yang, YL ;

Wang, ED ;

Xie, Y ;

Zhao, GP ;

Jiang, WH .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (05) :1298-1303

[20] EXPERIMENTAL EVOLUTION OF PENICILLIN-G ACYLASES FROM ESCHERICHIA-COLI AND PROTEUS-RETTGERI [J].

DAUMY, GO ;

DANLEY, D ;

MCCOLL, AS ;

APOSTOLAKOS, D ;

VINICK, FJ .

JOURNAL OF BACTERIOLOGY, 1985, 163 (03) :925-932

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