Development of an amperometric immunosensor for detection of staphylococcal enterotoxin type A in cheese

被引：34

作者：

Ribeiro Pimenta-Martins, Maria Gardenny ^{[1
]}

Furtado, Roselayne Ferro ^{[2
]}

Dias Heneine, Luiz Guilherme ^{[3
]}

Dias, Ricardo Souza ^{[3
]}

Borges, Maria de Fatima ^{[2
]}

Alves, Carlucio Roberto ^{[1
]}

机构：

[1] Univ Estadual Ceara, Dept Chem, Fortaleza, Ceara, Brazil

[2] Embrapa Trop Agroind, Fortaleza, Ceara, Brazil

[3] Ezequiel Dias Fdn FUNED, Belo Horizonte, MG, Brazil

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2012年 / 91卷 / 01期

关键词：

Food safety; Biosensor; Staphylococcal enterotoxin A; QUARTZ-CRYSTAL MICROBALANCE; SELF-ASSEMBLED MONOLAYERS; BIOSENSOR; AUREUS; IMMOBILIZATION; GOLD; FOOD; PEROXIDASE; SEPARATION; PROTEINS;

D O I：

10.1016/j.mimet.2012.05.016

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This paper reports a novel electrochemical immunosensor for the sensitive detection of staphylococcal enterotoxin A (SEA) based on self-assembly monolayer (SAM) and protein A immobilization on gold electrode. Three different methods of protein A immobilization were tested: physical adsorption, cross-linking using glutaraldehyde and covalent binding after activation with N-hydroxysuccinimide (NHS)/N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) on cysteamine-modified gold electrode. The EDC/NHS method for protein A immobilization was selected to lead development of the biosensor. The coating steps of the surface modification were characterized by cyclic voltammetly and the biosensor response by chronoamperometry. The advantages of the immunosensor were exposed in its high sensitivity and specificity. The proposed amperometric immunosensor was successfully used for determination of SEA in contaminated and non-contaminated cheese samples with excellent responses. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：138 / 143

页数：6

共 40 条

[1]

[Anonymous], 1990, OFFICIAL METHODS ANA

[2] COUPLING OF ENZYMES TO PROTEINS WITH GLUTARALDEHYDE . USE OF CONJUGATES FOR DETECTION OF ANTIGENS AND ANTIBODIES [J].

AVRAMEAS, S .

IMMUNOCHEMISTRY, 1969, 6 (01) :43-+

[3] Prevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Region of Turkey [J].

Aydin, Ali ;

Sudagidan, Mert ;

Muratoglu, Karlo .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2011, 148 (02) :99-106

[4] Evaluation of antibody immobilization methods for piezoelectric biosensor application [J].

Babacan, S ;

Pivarnik, P ;

Letcher, S ;

Rand, AG .

BIOSENSORS & BIOELECTRONICS, 2000, 15 (11-12) :615-621

[5] Staphylococcal enterotoxins [J].

Balaban, N ;

Rasooly, A .

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2000, 61 (01) :1-10

[6]

Bennett R.W., 2001, FDA BACTERIOLOGICAL

[7] Pomegranate extract inhibits Staphylococcus aureus growth and subsequent enterotoxin production [J].

Braga, LC ;

Shupp, JW ;

Cummings, C ;

Jett, M ;

Takahashi, JA ;

Carmo, LS ;

Chartone-Souza, E ;

Nascimento, AMA .

JOURNAL OF ETHNOPHARMACOLOGY, 2005, 96 (1-2) :335-339

[8] Olefin cross-metathesis of a vinyl-terminated self-assembled monolayer (SAM) on Au(111): Electrochemical study using a ferrocenyl redox center [J].

Brooksby, Paula A. ;

Anderson, Kelly H. ;

Downard, Alison J. ;

Abell, Andrew D. .

LANGMUIR, 2006, 22 (22) :9304-9312

[9] Characterization of alkanethiol-self-assembled monolayers-modified gold electrodes by electrochemical impedance spectroscopy [J].

Campuzano, S ;

Pedrero, M ;

Montemayor, C ;

Fatás, E ;

Pingarrón, JM .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2006, 586 (01) :112-121

[10] CULTURE MEDIUM FOR PRODUCTION OF STAPHYLOCOCCAL ENTEROTOXIN A [J].

CASMAN, EP ;

BENNETT, RW .

JOURNAL OF BACTERIOLOGY, 1963, 86 (01) :18-&

← 1 2 3 4 →