Antiproliferative activity of the Netherlands propolis and its active principles in cancer cell lines

被引：133

作者：

Banskota, AH ^{[1
]}

Nagaoka, T ^{[1
]}

Sumioka, LY ^{[1
]}

Tezuka, Y ^{[1
]}

Awale, S ^{[1
]}

Midorikawa, K ^{[1
]}

Matsushige, K ^{[1
]}

Kadota, S ^{[1
]}

机构：

[1] Toyama Med & Pharmaceut Univ, Inst Nat Med, Dept Nat Prod Chem, Toyama 9300194, Japan

来源：

JOURNAL OF ETHNOPHARMACOLOGY | 2002年 / 80卷 / 01期

关键词：

propolis; antiproliferative activity; DPPH radical scavenging activity; benzyl caffeate; phenethyl caffeate; cinnamyl caffeate;

D O I：

10.1016/S0378-8741(02)00022-3

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The MeOH extract of the Netherlands propolis showed promising antiproliferative activity toward highly liver-metastatic murine colon 26-L5 carcinoma with an EC50 value of 3.5 mug/ml. Further, antiproliferative activity-guided purification of the MeOH extract led us to isolate four flavonoids (1-4), seven cinnamic acid derivatives (5-11) and two new glycerol derivatives (12, 13), whose structures were elucidated on the basis of spectral analysis. The isolated compounds were tested for their antiproliferative activity against murine colon 26-L5, murine B16-BL6 melanoma, human HT-1080 fibrosarcoma and human lung A549 adenocarcinoma cell lines. The benzyl (9). values of, phenethyl (10) and cinnamyl caffeates (11) possessed potent antiproliferative activities with EC50 0.288, 1.76 and 0.114 muM, respectively, toward colon 26-L5 carcinoma. These caffeates were considered to be active constituents of the Netherlands propolis in their antiproliferative activity. The antioxidative activity of these caffeates may play an important role in their antiproliferative activities. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：67 / 73

页数：7

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