The human muscle proteome in aging

被引：126

作者：

Gelfi, Cecilia ^{[1
]}

Vigano, Agnese

Ripamonti, Marilena

Pontoglio, Alessandro

Begum, Shajna

Pellegrino, Maria Antonietta

Grassi, Bruno

Bottinelli, Roberto

Wait, Robin

Cerretelli, Paolo

机构：

[1] CNR, Inst Mol Bioimaging & Physiol, Segrate, MI, Italy

[2] Univ Milan, Dept Biomed Sci & Technol, I-20133 Milan, Italy

[3] Univ London Imperial Coll Sci Technol & Med, Kennedy Inst Rheumatol, London, England

[4] Univ Pavia, Dept Expt Med, Human Physiol Unit, I-27100 Pavia, Italy

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 06期

关键词：

aging; myosin; phosphorylation; 2-D DIGE; mass spectrometry;

D O I：

10.1021/pr050414x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of the present study was to assess age-dependent changes of proteins in the vastus lateralis muscle of physically active elderly and young subjects by a combination of two-dimensional difference gel electrophoresis, SDS-PAGE and ESI-MS/MS. The differences observed in the elderly group included down-regulation of regulatory myosin light chains, particularly the phosphorylated isoforms, a higher proportion of myosin heavy chain isoforms 1 and 2A, and enhanced oxidative and reduced glycolytic capacity.

引用

页码：1344 / 1353

页数：10

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