Adipose tissue stearoyl-CoA desaturase mRNA is increased by obesity and decreased by polyunsaturated fatty acids

被引:75
作者
Jones, BH
Maher, MA
Banz, WJ
Zemel, MB
Whelan, J
Smith, PJ
Moustaid, N
机构
[1] UNIV TENNESSEE, DEPT NUTR, KNOXVILLE, TN 37996 USA
[2] UNIV TENNESSEE, PHYSIOL PROGRAM, KNOXVILLE, TN 37996 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM | 1996年 / 271卷 / 01期
关键词
3T3-L1; adipocytes; Zucker rats; linoleate;
D O I
10.1152/ajpendo.1996.271.1.E44
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Stearoyl-CoA desaturase (SCD) is a key regulatory enzyme in the synthesis of unsaturated fatty acids. Although regulation of hepatic SCD by obesity and polyunsaturated fatty acids (PUFA) has been well investigated, no studies have addressed whether similar regulation occurs in adipose tissue. We addressed these questions by feeding control (12% corn oil) and high-PUFA (48% corn oil) diets to lean and obese Zucker rats and analyzing SCD mRNA levels in adipose tissue and liver. We report that SCD mRNA content was dramatically elevated in adipose tissue of obese vs. lean rats on both diets and was significantly decreased by PUFA in both genotypes. Interestingly, we demonstrate that SCD expression was directly downregulated in a dose-dependent manner by PUFA in 3T3-L1 adipocytes. We conclude that 1) obese Zucker rats overexpress the SCD gene in both liver and adipose tissue and 2) PUFA directly suppress SCD expression in adipocytes. Further studies will elucidate the mechanisms responsible for obesity- and PUFA-mediated regulation of SCD in adipose cells.
引用
收藏
页码:E44 / E49
页数:6
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