Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells

被引:96
作者
Ghildyal, R
Mills, J
Murray, M
Vardaxis, N
Meanger, J
机构
[1] Macfarlane Burnet Inst Med Res & Publ Hlth, Childrens Virol Res Unit, Fairfield, Vic 3078, Australia
[2] RMIT Univ, Sch Med Sci, Dept Med Lab Sci, Bundoora, Vic 3083, Australia
关键词
D O I
10.1099/0022-1317-83-4-753
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Little is known about the functions of the matrix (M) protein of respiratory syncytial virus (RSV). By analogy with other negative-strand RNA viruses, the M protein should inhibit the viral polymerase prior to packaging and facilitate virion assembly. In this study, localization of the RSV M protein in infected cells and its association with the RSV nucleocapsid complex was investigated. RSV-infected cells were shown to contain characteristic cytoplasmic inclusions. Further analysis showed that these inclusions were localization sites of the M protein as well as the N, P, L and M2-1 proteins described previously. The M protein co-purified with viral ribonucleoproteins (RNPs) from RSV-infected cells. The transcriptase activity of purified RNPs was enhanced by treatment with antibodies to the M protein in a dose-dependent manner. These data suggest that the M protein is associated with RSV nucleocapsids and, like the matrix proteins of other negative-strand RNA viruses, can inhibit virus transcription.
引用
收藏
页码:753 / 757
页数:5
相关论文
共 24 条
[1]   The NS1 protein of human respiratory syncytial virus is a potent inhibitor of minigenome transcription and RNA replication [J].
Atreya, PL ;
Peeples, ME ;
Collins, PL .
JOURNAL OF VIROLOGY, 1998, 72 (02) :1452-1461
[3]   The M2-2 protein of human respiratory syncytial virus is a regulatory factor involved in the balance between RNA replication and transcription [J].
Bermingham, A ;
Collins, PL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (20) :11259-11264
[4]   Profilin is required for optimal actin-dependent transcription of respiratory syncytial virus genome RNA [J].
Burke, E ;
Mahoney, NM ;
Almo, SC ;
Barik, S .
JOURNAL OF VIROLOGY, 2000, 74 (02) :669-675
[5]   ROLE OF THE MEMBRANE (M) PROTEIN IN ENDOGENOUS INHIBITION OF INVITRO TRANSCRIPTION BY VESICULAR STOMATITIS-VIRUS [J].
CARROLL, AR ;
WAGNER, RR .
JOURNAL OF VIROLOGY, 1979, 29 (01) :134-142
[6]  
Collins P.L., 1996, FIELDS VIROLOGY, V3, P1313
[7]   Nucleocapsid incorporation into parainfluenza virus is regulated by specific interaction with matrix protein [J].
Coronel, EC ;
Takimoto, T ;
Murti, KG ;
Varich, N ;
Portner, A .
JOURNAL OF VIROLOGY, 2001, 75 (03) :1117-1123
[8]   PARAMYXOVIRUS PHOSPHOPROTEINS FORM HOMOTRIMERS AS DETERMINED BY AN EPITOPE DILUTION ASSAY, VIA PREDICTED COILED COILS [J].
CURRAN, J ;
BOECK, R ;
LINMARQ, N ;
LUPAS, A ;
KOLAKOFSKY, D .
VIROLOGY, 1995, 214 (01) :139-149
[9]   HUMAN PARAINFLUENZA VIRUS TYPE-3 TRANSCRIPTION INVITRO - ROLE OF CELLULAR ACTIN IN MESSENGER-RNA SYNTHESIS [J].
DE, BP ;
LESOON, A ;
BANERJEE, AK .
JOURNAL OF VIROLOGY, 1991, 65 (06) :3268-3275
[10]   CYTOPLASMIC INCLUSIONS OF RESPIRATORY SYNCYTIAL VIRUS-INFECTED CELLS - FORMATION OF INCLUSION-BODIES IN TRANSFECTED CELLS THAT COEXPRESS THE NUCLEOPROTEIN, THE PHOSPHOPROTEIN, AND THE 22K PROTEIN [J].
GARCIA, J ;
GARCIABARRENO, B ;
VIVO, A ;
MELERO, JA .
VIROLOGY, 1993, 195 (01) :243-247