BRAHMA ATPase of the SWI/SNF Chromatin Remodeling Complex Acts as a Positive Regulator of Gibberellin-Mediated Responses in Arabidopsis

被引:64
作者
Archacki, Rafal [1 ]
Buszewicz, Daniel [2 ]
Sarnowski, Tomasz J. [2 ]
Sarnowska, Elzbieta [3 ]
Rolicka, Anna T. [1 ]
Tohge, Takayuki [4 ]
Fernie, Alisdair R. [4 ]
Jikumaru, Yusuke [5 ]
Kotlinski, Maciej [2 ]
Iwanicka-Nowicka, Roksana [1 ,2 ]
Kalisiak, Katarzyna [2 ]
Patryn, Jacek [1 ]
Halibart-Puzio, Joanna [2 ]
Kamiya, Yuji [5 ]
Davis, Seth J. [3 ]
Koblowska, Marta K. [1 ,2 ]
Jerzmanowski, Andrzej [1 ,2 ]
机构
[1] Univ Warsaw, Fac Biol, Dept Plant Mol Biol, Warsaw, Poland
[2] Polish Acad Sci, Inst Biochem & Biophys, Warsaw, Poland
[3] Max Planck Inst Plant Breeding, Cologne, Germany
[4] Max Planck Inst Mol Plant Physiol, Potsdam, Germany
[5] RIKEN, Plant Sci Ctr, Tsurumi Ku, Yokohama, Kanagawa, Japan
来源
PLOS ONE | 2013年 / 8卷 / 03期
关键词
SCARECROW-LIKE; 3; FLORAL DEVELOPMENT; SEED-GERMINATION; GENETIC-ANALYSIS; DELLA; GROWTH; PLANT; THALIANA; ROLES; EXPRESSION;
D O I
10.1371/journal.pone.0058588
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
SWI/SNF chromatin remodeling complexes perform a pivotal function in the regulation of eukaryotic gene expression. Arabidopsis (Arabidopsis thaliana) mutants in major SWI/SNF subunits display embryo-lethal or dwarf phenotypes, indicating their critical role in molecular pathways controlling development and growth. As gibberellins (GA) are major positive regulators of plant growth, we wanted to establish whether there is a link between SWI/SNF and GA signaling in Arabidopsis. This study revealed that in brm-1 plants, depleted in SWI/SNF BRAHMA (BRM) ATPase, a number of GA-related phenotypic traits are GA-sensitive and that the loss of BRM results in markedly decreased level of endogenous bioactive GA. Transcriptional profiling of brm-1 and the GA biosynthesis mutant ga1-3, as well as the ga1-3/brm-1 double mutant demonstrated that BRM affects the expression of a large set of GA-responsive genes including genes responsible for GA biosynthesis and signaling. Furthermore, we found that BRM acts as an activator and directly associates with promoters of GA3ox1, a GA biosynthetic gene, and SCL3, implicated in positive regulation of the GA pathway. Many GA-responsive gene expression alterations in the brm-1 mutant are likely due to depleted levels of active GAs. However, the analysis of genetic interactions between BRM and the DELLA GA pathway repressors, revealed that BRM also acts on GA-responsive genes independently of its effect on GA level. Given the central position occupied by SWI/SNF complexes within regulatory networks controlling fundamental biological processes, the identification of diverse functional intersections of BRM with GA-dependent processes in this study suggests a role for SWI/SNF in facilitating crosstalk between GA-mediated regulation and other cellular pathways.
引用
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页数:12
相关论文
共 70 条
[51]   SWI3 subunits of putative SWI/SNF chromatin-remodeling complexes play distinct roles during Arabidopsis development [J].
Sarnowski, TJ ;
Ríos, G ;
Jásik, J ;
Swiezewski, S ;
Kaczanowski, S ;
Li, Y ;
Kwiatkowska, A ;
Pawlikowska, K ;
Kozbial, M ;
Kozbial, P ;
Koncz, C ;
Jerzmanowski, A .
PLANT CELL, 2005, 17 (09) :2454-2472
[52]   Understanding gibberellic acid signaling - are we there yet? [J].
Schwechheimer, Claus .
CURRENT OPINION IN PLANT BIOLOGY, 2008, 11 (01) :9-15
[53]  
Silverstone AL, 1997, GENETICS, V146, P1087
[54]   The Arabidopsis RGA gene encodes a transcriptional regulator repressing the gibberellin signal transduction pathway [J].
Silverstone, AL ;
Ciampaglio, CN ;
Sun, TP .
PLANT CELL, 1998, 10 (02) :155-169
[55]   Functional analysis of SPINDLY in gibberellin signaling in Arabidopsis [J].
Silverstone, Aron L. ;
Tseng, Tong-Seung ;
Swain, Stephen M. ;
Dill, Alyssa ;
Jeong, Sun Yong ;
Olszewski, Neil E. ;
Sun, Tai-ping .
PLANT PHYSIOLOGY, 2007, 143 (02) :987-1000
[56]   The Molecular Mechanism and Evolution of the GA-GID1-DELLA Signaling Module in Plants [J].
Sun, Tai-ping .
CURRENT BIOLOGY, 2011, 21 (09) :R338-R345
[57]  
Sun Tai-Ping, 2008, Arabidopsis Book, V6, pe0103, DOI 10.1199/tab.0103
[58]   ENDOGENOUS GIBBERELLINS IN ARABIDOPSIS-THALIANA AND POSSIBLE STEPS BLOCKED IN THE BIOSYNTHETIC PATHWAYS OF THE SEMIDWARF GA4 AND GA5 MUTANTS [J].
TALON, M ;
KOORNNEEF, M ;
ZEEVAART, JAD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (20) :7983-7987
[59]   The Arabidopsis BRAHMA chromatin-remodeling ATPase is involved in repression of seed maturation genes in leaves [J].
Tang, Xurong ;
Hou, Anfu ;
Babu, Mohan ;
Nguyen, Vi ;
Hurtado, Lidia ;
Lu, Qing ;
Reyes, Jose C. ;
Wang, Aiming ;
Keller, Wilfred A. ;
Harada, John J. ;
Tsang, Edward W. T. ;
Cui, Yuhai .
PLANT PHYSIOLOGY, 2008, 147 (03) :1143-1157
[60]   DELLA proteins and gibberellin-regulated seed germination and floral development in Arabidopsis [J].
Tyler, L ;
Thomas, SG ;
Hu, JH ;
Dill, A ;
Alonso, JM ;
Ecker, JR ;
Sun, TP .
PLANT PHYSIOLOGY, 2004, 135 (02) :1008-1019