Interaction of penicillin V acylase with organic solvents:: catalytic activity modulation on the hydrolysis of penicillin V

被引：29

作者：

Arroyo, M ^{[1
]}

Torres, R ^{[1
]}

de la Mata, I ^{[1
]}

Castillón, MP ^{[1
]}

Acebal, C ^{[1
]}

机构：

[1] Univ Complutense Madrid, Fac Ciencias Biol, Dept Bioquim & Biol Mol 1, E-28040 Madrid, Spain

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1999年 / 25卷 / 3-5期

关键词：

penicillin V acylase; hydrolysis; organic solvents; dielectric constant; enzyme denaturation;

D O I：

10.1016/S0141-0229(99)00057-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The modulation of hydrolytic activity of penicillin V acylase (EC 3.5.1.11) from Streptomyces lavendulae by organic solvents is reported. On one hand, the addition of water-soluble cosolvents increases the catalytic activity up to a critical concentration of the non-aqueous solvents, yet further increase of the latter leads to protein denaturation. For alcohols and aprotic polar solvents, there are linear correlations between the critical concentration of water miscible cosolvent (at which enzyme deactivation does not begin to take place) and the dielectric constant of the cosolvents added. On the other hand, water-immiscible solvents can show activating or inhibitory effects that may be related to interactions between the structure of the solvent and the enzyme. (C) 1999 Elsevier Science Inc. All rights reserved.

引用

页码：378 / 383

页数：6

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