Simultaneous determination of catecholamines in rat brain tissue by high-performance liquid chromatography

被引：47

作者：

Chi, JD ^{[1
]}

Odontiadis, J ^{[1
]}

Franklin, M ^{[1
]}

机构：

[1] Univ Oxford, Warneford Hosp, Dept Psychiat, Psychopharmacol Res Unit, Oxford OX3 7JX, England

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 731卷 / 02期

关键词：

catecholamines; norepinephrine; dopamine; serotonin;

D O I：

10.1016/S0378-4347(99)00255-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel and highly sensitive method has been developed for the determination of catecholamines [noradrenaline (NA), dopamine (DA), serotonin (5-HT) and their metabolites 5-hydroxyindoleacetic acid (5-HIAA) and homovanillic acid (HVA)] in brain tissue. The method uses isocratic reversed-phase HPLC with amperometric end-point detection. The calibration curve was linear over the range 10-150 pg on-column. The assay limits of detection for NA, DA, 5-MT, 5-HIAA and HVA were 3.8, 3.8, 6.8, 5 and 7.5 pg on-column, respectively. The mean inter- and intra-assay relative standard deviations (RSDs) over the range of the standard curve were less than 5%. The absolute recoveries averaged 99.1%, 99.5%, 97.7%, 99.5% and 98.8% for NA, DA, 5-HT, 5-HIAA and HVA, respectively. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：361 / 367

页数：7

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