Antibodies are forever: a study using 12-26-year-old expired antibodies

被引：20

作者：

Argentieri, Maria C. ^{[1
]}

Pilla, Daniela ^{[1
]}

Vanzati, Alice ^{[1
,2
]}

Lonardi, Silvia ^{[3
]}

Facchetti, Fabio ^{[3
]}

Doglioni, Claudio ^{[4
]}

Parravicini, Carlo ^{[5
]}

Cattoretti, Giorgio ^{[1
,2
]}

机构：

[1] AO San Gerardo, Dept Pathol, Monza, Italy

[2] Univ Milano Bicocca, Dept Surg Sci, Milan, Italy

[3] Univ Brescia, Spedali Civili, Dept Pathol, Brescia, Italy

[4] Hosp San Raffaele, Surg Pathol Unit, I-20132 Milan, Italy

[5] Osped L Sacco, Dept Pathol, Milan, Italy

来源：

HISTOPATHOLOGY | 2013年 / 63卷 / 06期

关键词：

antibody; expiry date; immunohistochemistry; MONOCLONAL-ANTIBODY; CELL-PROLIFERATION; NUCLEAR ANTIGEN; T-CELL; B-CELL; EXPRESSION; EXPIRATION;

D O I：

10.1111/his.12225

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

AimsThe aim of this study was to investigate whether the shelf-life of diagnostic antibodies is longer than the expiry date on the label. Methods and resultsFour independent laboratories tested a small number of diagnostic antibodies kept at +4 degrees C for 12-26years, and found them to work perfectly on routine histology sections. ConclusionsDiagnostic antibodies may have a workable half-life in excess of 10years, and the emphasis on performance should shift to the preservation of antigenic targets in the tissue.

引用

页码：869 / 876

页数：8

共 31 条

[11]

Kent U M, 1999, Methods Mol Biol, V115, P11

[12] EPITOPE ANALYSIS OF ANTIBODIES RECOGNIZING THE CELL-PROLIFERATION ASSOCIATED NUCLEAR ANTIGEN PREVIOUSLY DEFINED BY THE ANTIBODY KI-67 (KI-67 PROTEIN) [J].