Germline excision of transgenes in Aedes aegypti by homing endonucleases

被引：25

作者：

Aryan, Azadeh

Anderson, Michelle A. E.

Myles, Kevin M.

Adelman, Zach N. ^{[1
]}

机构：

[1] Virginia Tech, Fralin Life Sci Inst, Blacksburg, VA 24061 USA

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

YELLOW-FEVER MOSQUITO; DIRECTED EVOLUTION; ANOPHELES-GAMBIAE; DNA BREAKS; GENE; RECOMBINATION; DROSOPHILA; DERIVATIVES; POPULATION; SEQUENCES;

D O I：

10.1038/srep01603

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Aedes (Ae.) aegypti is the primary vector for dengue viruses (serotypes1-4) and chikungunya virus. Homing endonucleases (HEs) are ancient selfish elements that catalyze double-stranded DNA breaks (DSB) in a highly specific manner. In this report, we show that the HEs Y2-I-AniI, I-CreI and I-SceI are all capable of catalyzing the excision of genomic segments from the Ae. aegypti genome in a heritable manner. Y2-I-AniI demonstrated the highest efficiency at two independent genomic targets, with 20-40% of Y2-I-AniI-treated individuals producing offspring that had lost the target transgene. HE-induced DSBs were found to be repaired via the single-strand annealing (SSA) and non-homologous end-joining (NHEJ) pathways in a manner dependent on the availability of direct repeat sequences in the transgene. These results support the development of HE-based gene editing and gene drive strategies in Ae. aegypti, and confirm the utility of HEs in the manipulation and modification of transgenes in this important vector.

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页数：8

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