Positions of strand exchange in mycobacteriophage L5 integration and characterization of the attB site

被引：41

作者：

Pena, CEA ^{[1
]}

Stoner, JE ^{[1
]}

Hatfull, GF ^{[1
]}

机构：

[1] UNIV PITTSBURGH,DEPT BIOL SCI,PITTSBURGH,PA 15260

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 18期

关键词：

D O I：

10.1128/jb.178.18.5533-5536.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacteriophage L5 integrates into the genome of Mycobacterium smegmatis via site-specific recombination between the phage attP site and the bacterial attB site. These two sites have a 43-bp common core sequence within which strand exchange occurs and which overlaps a tRNA(Gly) gene at attB, We show here that a 29-bp segment of DNA is necessary and sufficient for attB function and identify the positions of strand exchange.

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页码：5533 / 5536

页数：4

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