Lipid composition of the gram-negative, budding, seawater bacterium Hyphomonas jannaschiana lacking in phospholipids

被引:14
作者
Batrakov, SG [1 ]
Nikitin, DI [1 ]
Pitryuk, IA [1 ]
机构
[1] RUSSIAN ACAD SCI,INST MICROBIOL,MOSCOW 117811,RUSSIA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM | 1996年 / 1303卷 / 01期
关键词
glycolipid; glucosyldiacylglycerol; glucuronosyldiacylglycerol; glucuronic acid acylated; mass spectrometry; (H-jannaschiana);
D O I
10.1016/0005-2760(96)00072-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The extractable polar lipids of the gram-negative, budding, seawater bacterium Hyphomonas jannaschiana VP-2(T) were shown to of five glycolipids, namely, 1,2-diacyl-3-alpha-D-glucopyranosyl-sn-glycerol (I) (25% of the total lipids), 1,2-diacyl-3-alpha-D-glucuronopyranosyl-sn-glycerol (II) (41%), taurine-amide of II (III) (32%), acyl-D-glucuronopyranoside (IV) (0.9%), and acyl-3-O-acyl-D-glucuronopyranoside (V) (0.4%); glycolipid (III) was described by the authors previously. In addition, small amounts of poly(beta-hydroxybutyrate) and free fatty acids were present in the Lipids extracted. Neither phospholipids nor low polarity lipids, such as fatty acid esters of glycerol or fatty alcohols, were detected. Glycolipids I, II, IV, V were isolated in chromatographically pure state by chromatography on DEAE-cellulose and silica-gel columns. Their structures were established using chromatographic, spectrometric and chemical-degradation methods. In glyceroglycolipids I and II, unsaturated fatty acid residues were located predominantly at the sn-1 carbon of the glycerol moiety while saturated ones were linked mainly to the sn-2 carbon.
引用
收藏
页码:39 / 46
页数:8
相关论文
共 26 条
[1]  
BATRAKOV SG, 1975, BIOORG KHIM+, V1, P725
[2]   A novel glycolipid 1,2-diacyl-3-alpha-D-glucuronopyranosyl-sn-glycerol taurineamide, from the budding seawater bacterium Hyphomonas jannaschiana [J].
Batrakov, SG ;
Nikitin, DI ;
Pitryuk, IA .
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1996, 1302 (02) :167-176
[3]   MOLECULAR-SPECIES ANALYSIS OF LIPIDS BY METASTABLE ION MASS-SPECTROMETRY [J].
BATRAKOV, SG ;
SADOVSKAYA, VL ;
ROZYNOV, BV ;
BERGELSON, LD .
CHEMISTRY AND PHYSICS OF LIPIDS, 1983, 33 (04) :331-353
[4]  
BATRAKOV SG, 1975, IAN SSSR KH, P94
[5]  
BATRAKOV SG, 1975, IAN SSSR KH, P821
[6]  
BATRAKOV SG, 1976, IZV ANK SSSR K, P643
[7]  
BATRAKOV SG, 1974, IAN SSSR KH, P2229
[8]   APPLICATION OF MASS SPECTROMETRY TO STRUCTURE PROBLEMS .13. ACETATES OF PENTOSES AND HEXOSES [J].
BIEMANN, K ;
SCHNOES, HK ;
DEJONGH, DC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (12) :1763-&
[9]  
BUDZIKIE.H, 1973, Z NATURFORSCH C, VC 28, P499
[10]   APPLICATION OF MASS SPECTROMETRY TO STRUCTURE PROBLEMS .14. ACETATES OF PARTIALLY METHYLATEDPENTOSES AND HEXOSES [J].
DEJONGH, DC ;
BIEMANN, K .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1963, 85 (15) :2289-&