Lipid composition of the gram-negative, budding, seawater bacterium Hyphomonas jannaschiana lacking in phospholipids

The extractable polar lipids of the gram-negative, budding, seawater bacterium Hyphomonas jannaschiana VP-2(T) were shown to of five glycolipids, namely, 1,2-diacyl-3-alpha-D-glucopyranosyl-sn-glycerol (I) (25% of the total lipids), 1,2-diacyl-3-alpha-D-glucuronopyranosyl-sn-glycerol (II) (41%), taurine-amide of II (III) (32%), acyl-D-glucuronopyranoside (IV) (0.9%), and acyl-3-O-acyl-D-glucuronopyranoside (V) (0.4%); glycolipid (III) was described by the authors previously. In addition, small amounts of poly(beta-hydroxybutyrate) and free fatty acids were present in the Lipids extracted. Neither phospholipids nor low polarity lipids, such as fatty acid esters of glycerol or fatty alcohols, were detected. Glycolipids I, II, IV, V were isolated in chromatographically pure state by chromatography on DEAE-cellulose and silica-gel columns. Their structures were established using chromatographic, spectrometric and chemical-degradation methods. In glyceroglycolipids I and II, unsaturated fatty acid residues were located predominantly at the sn-1 carbon of the glycerol moiety while saturated ones were linked mainly to the sn-2 carbon.