Alterations of Fas (Apo-1/CD95) gene in cutaneous malignant melanoma

被引:113
作者
Shin, MS
Park, WS
Kim, SY
Kim, HS
Kang, SJ
Song, KY
Park, JY
Dong, SM
Pi, JH
Oh, RR
Lee, JY
Yoo, NJ
Lee, SH
机构
[1] Catholic Univ Korea, Coll Med, Dept Pathol, Socho Gu, Seoul 137701, South Korea
[2] Catholic Univ Korea, Coll Med, Dept Biochem, Seoul 137701, South Korea
[3] Catholic Univ Korea, Coll Med, Dept Clin Pathol, Seoul 137701, South Korea
[4] Catholic Univ Korea, Coll Med, Canc Res Inst, Seoul 137701, South Korea
[5] Chung Ang Univ, Coll Med, Dept Pathol, Seoul 156756, South Korea
关键词
D O I
10.1016/S0002-9440(10)65434-X
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Fas (Apo-1/CD95) is a cell-surface receptor involved in cell death signaling, The key role of the Fas system in negative growth regulation has been studied mostly within the immune system, and somatic mutations of Fas gene in cancer patients have been described solely in lymphoid-lineage malignancies, However, many nonlymphoid tumor cells have been found to be resistant to Fas-mediated apoptosis, which suggests that Fas mutations, one of the possible mechanisms for Fas resistance, may be involved in the pathogenesis of nonlymphoid malignancies as well. In this study, we have analyzed the entire coding region and all splice sites of the Fas gene for the detection of the gene mutations in 44 human malignant melanomas in skin by polymerase chain reaction, single-strand conformation polymorphism, and DNA sequencing. Overall, 3 tumors (6.8%) were found to have the Fns mutations, which were all missense variants and identified in the cytoplasmic region (death domain) known to be involved in the transduction of an apoptotic signal, The data presented here suggest that somatic alterations of the Fns gene might Lad to the loss of its apoptotic function and contribute to the pathogenesis of some human malignant melanomas.
引用
收藏
页码:1785 / 1791
页数:7
相关论文
共 41 条
  • [21] A simple, precise and economical microdissection technique for analysis of genomic DNA from archival tissue sections
    Lee, JY
    Dong, SM
    Kim, SY
    Yoo, NJ
    Lee, SH
    Park, WS
    [J]. VIRCHOWS ARCHIV, 1998, 433 (04) : 305 - 309
  • [22] Lee SH, 1998, LAB INVEST, V78, P453
  • [23] LEITHAUSER F, 1993, LAB INVEST, V69, P415
  • [24] Apoptosis by death factor
    Nagata, S
    [J]. CELL, 1997, 88 (03) : 355 - 365
  • [25] Lack of cell surface Fas/APO-1 expression in pulmonary adenocarcinomas
    Nambu, Y
    Hughes, SJ
    Rehemtulla, A
    Hamstra, D
    Orringer, MB
    Beer, DG
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (05) : 1102 - 1110
  • [26] NATOLI G, 1995, ONCOGENE, V11, P1157
  • [27] DISTRIBUTION OF APOPTOSIS-MEDIATING FAS ANTIGEN IN HUMAN SKIN AND EFFECTS OF ANTI-FAS MONOCLONAL-ANTIBODY ON HUMAN EPIDERMAL KERATINOCYTE AND SQUAMOUS-CELL CARCINOMA CELL-LINES
    OISHI, M
    MAEDA, K
    SUGIYAMA, S
    [J]. ARCHIVES OF DERMATOLOGICAL RESEARCH, 1994, 286 (07) : 396 - 407
  • [28] OWENSCHAUB LB, 1994, CANCER RES, V54, P1580
  • [29] MUTATIONS IN FAS ASSOCIATED WITH HUMAN LYMPHOPROLIFERATIVE SYNDROME AND AUTOIMMUNITY
    RIEUXLAUCAT, F
    LEDEIST, F
    HIVROZ, C
    ROBERTS, IAG
    DEBATIN, KM
    FISCHER, A
    DEVILLARTAY, JP
    [J]. SCIENCE, 1995, 268 (5215) : 1347 - 1349
  • [30] Rivoltini L, 1998, J IMMUNOL, V161, P1220