Transgenic mice with defined combinations of drug-inducible reprogramming factors

被引：79

作者：

Markoulaki, Styliani ^{[1
]}

Hanna, Jacob ^{[1
]}

Beard, Caroline ^{[1
]}

Carey, Bryce W. ^{[1
,2
]}

Cheng, Albert W. ^{[1
,2
]}

Lengner, Christopher J. ^{[1
]}

Dausman, Jessica A. ^{[1
]}

Fu, Dongdong ^{[1
]}

Gao, Qing ^{[1
]}

Wu, Su ^{[1
]}

Cassady, John P. ^{[1
,2
]}

Jaenisch, Rudolf ^{[1
,2
]}

机构：

[1] Whitehead Inst Biomed Res, Cambridge, MA 02142 USA

[2] MIT, Dept Biol, Cambridge, MA 02142 USA

来源：

NATURE BIOTECHNOLOGY | 2009年 / 27卷 / 02期

基金：

美国国家卫生研究院;

关键词：

PLURIPOTENT STEM-CELLS; HUMAN FIBROBLASTS; MOUSE; INDUCTION; OCT4; MYC;

D O I：

10.1038/nbt.1520

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Proviruses carrying drug-inducible Oct4, Sox2, Klf4 and c-Myc used to derive 'primary' induced pluripotent stem (iPS) cells were segregated through germline transmission, generating mice and cells carrying subsets of the reprogramming factors. Drug treatment produced 'secondary' iPS cells only when the missing factor was introduced. This approach creates a defined system for studying reprogramming mechanisms and allows screening of genetically homogeneous cells for compounds that can replace any transcription factor required for iPS cell derivation.

引用

页码：169 / 171

页数：3