Structure of the uncleaved human H1 hemagglutinin from the extinct 1918 influenza virus

被引:375
作者
Stevens, J
Corper, AL
Basler, CF
Taubenberger, JK
Palese, P
Wilson, IA
机构
[1] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA
[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA
[3] Mt Sinai Sch Med, Dept Microbiol, New York, NY 10029 USA
[4] Armed Forces Inst Pathol, Dept Cellular Pathol & Genet, Div Mol Pathol, Washington, DC 20306 USA
关键词
D O I
10.1126/science.1093373
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The 1918 "Spanish" influenza pandemic represents the largest recorded outbreak of any infectious disease. The crystal structure of the uncleaved precursor of the major surface antigen of the extinct 1918 virus was determined at 3.0 angstrom resolution after reassembly of the hemagglutinin gene from viral RNA fragments preserved in 1918 formalin-fixed lung tissues. A narrow avian-like receptor-binding site, two previously unobserved histidine patches, and a less exposed surface loop at the cleavage site that activates viral membrane fusion reveal structural features primarily found in avian viruses, which may have contributed to the extraordinarily high infectivity and mortality rates observed during 1918.
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收藏
页码:1866 / 1870
页数:5
相关论文
共 39 条
  • [1] Sequence of the 1918 pandemic influenza virus nonstructural gene (NS) segment and characterization of recombinant viruses hearing the 1918 NS genes
    Basler, CF
    Reid, AH
    Dybing, JK
    Janczewski, TA
    Fanning, TG
    Zheng, HY
    Salvatore, M
    Perdue, ML
    Swayne, DE
    García-Sastre, A
    Palese, P
    Taubenberger, JK
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (05) : 2746 - 2751
  • [2] BEKLEMISHEV AB, 1993, MOL GEN MIKROBIOL VI, V1, P24
  • [3] Structure of influenza haemagglutinin at neutral and at fusogenic pH by electron cryo-microscopy
    Böttcher, C
    Ludwig, K
    Herrmann, A
    van Heel, M
    Stark, H
    [J]. FEBS LETTERS, 1999, 463 (03): : 255 - 259
  • [4] The predicted antigenicity of the haemagglutinin of the 1918 Spanish influenza pandemic suggests an avian origin
    Brownlee, GG
    Fodor, E
    [J]. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES, 2001, 356 (1416) : 1871 - 1876
  • [5] STRUCTURE OF INFLUENZA HEMAGGLUTININ AT THE PH OF MEMBRANE-FUSION
    BULLOUGH, PA
    HUGHSON, FM
    SKEHEL, JJ
    WILEY, DC
    [J]. NATURE, 1994, 371 (6492) : 37 - 43
  • [6] Membrane fusion induced by vesicular stomatitis virus depends on histidine protonation
    Carneiro, FA
    Stauffer, F
    Lima, CS
    Juliano, MA
    Juliano, L
    Da Poian, AT
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (16) : 13789 - 13794
  • [7] Structure of the hemagglutinin precursor cleavage site, a determinant of influenza pathogenicity and the origin of the labile conformation
    Chen, J
    Lee, KH
    Steinhauer, DA
    Stevens, DJ
    Skehel, JJ
    Wiley, DC
    [J]. CELL, 1998, 95 (03) : 409 - 417
  • [8] RECEPTOR SPECIFICITY IN HUMAN, AVIAN, AND EQUINE H2 AND H3 INFLUENZA-VIRUS ISOLATES
    CONNOR, RJ
    KAWAOKA, Y
    WEBSTER, RG
    PAULSON, JC
    [J]. VIROLOGY, 1994, 205 (01) : 17 - 23
  • [9] de Jong J C, 2003, Ned Tijdschr Geneeskd, V147, P1971
  • [10] Mechanism of acidification of the trans-Golgi network (TGN) -: In situ measurements of pH using retrieval of TGN38 and furin from the cell surface
    Demaurex, N
    Furuya, W
    D'Souza, S
    Bonifacino, JS
    Grinstein, S
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (04) : 2044 - 2051