Over-expression of the citrus gene CtNH1 confers resistance to bacterial canker disease

被引：20

作者：

Chen, Xiuhua ^{[1
]}

Barnaby, Jinyoung Y. ^{[2
]}

Sreedharan, Aswathy ^{[3
]}

Huang, Xiaoen ^{[1
]}

Orbovic, Vladimir ^{[3
]}

Grosser, Jude W. ^{[3
]}

Wang, Nian ^{[3
]}

Dong, Xinnian ^{[2
]}

Song, Wen-Yuan ^{[1
]}

机构：

[1] Univ Florida, Dept Plant Pathol, Plant Mol & Cellular Biol Program, Gainesville, FL 32611 USA

[2] Duke Univ, Dept Biol, Durham, NC 27708 USA

[3] Univ Florida, Citrus Res & Educ Ctr, Lake Alfred, FL 33850 USA

来源：

PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY | 2013年 / 84卷

基金：

美国国家科学基金会;

关键词：

NPR1; Transgenic plants; Systemic acquired resistance; Canker disease; Citrus; CONSTITUTIVE ACTIVATION; ENHANCED RESISTANCE; NPR1-LIKE GENES; SALICYLIC-ACID; ARABIDOPSIS; NPR1; OVEREXPRESSION; PROTEIN; MUTANT; RESPONSES;

D O I：

10.1016/j.pmpp.2013.07.002

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Citrus canker is a devastating disease, caused by Xanthomonas axonopodis pv. citri (Xac). It is well established that the NPR1 gene plays a pivotal role in systemic acquired resistance (SAR) in Arabidopsis. In this study, we report the isolation and characterization of an NPR1 homolog from citrus, namely Citrus NPR1 homolog 1 (CtNH1). Sequence alignment and phylogenetic analysis indicate that CtNH1 is closely-related to the Arabidopsis NPR1 gene and its orthologs from rice, grapevine, and cacao. When over-expressed in citrus, CtNH1 confers resistance to Xac and leads to constitutive expression of the pathogenesis-related (PR) gene chitinase 1 (Chi1), suggesting that CtNH1 is orthologous to NPR1. Published by Elsevier Ltd.

引用

页码：115 / 122

页数：8

共 38 条

[1] Xanthomonas citri:: breaking the surface [J].

Brunings, AM ;

Gabriel, DW .

MOLECULAR PLANT PATHOLOGY, 2003, 4 (03) :141-157

[2] Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance [J].

Cao, H ;

Li, X ;

Dong, XN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (11) :6531-6536

[3] The Arabidopsis NPR1 gene that controls systemic acquired resistance encodes a novel protein containing ankyrin repeats [J].

Cao, H ;

Glazebrook, J ;

Clarke, JD ;

Volko, S ;

Dong, XN .

CELL, 1997, 88 (01) :57-63

[4]

CAO H, 1994, PLANT CELL, V6, P1583, DOI 10.1105/tpc.6.11.1583

[5] Overexpression of a rice NPR1 homolog leads to constitutive activation of defense response and hypersensitivity to light [J].

Chern, M ;

Fitzgerald, HA ;

Canlas, PE ;

Navarre, DA ;

Ronald, PC .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2005, 18 (06) :511-520

[6] Evidence for a disease-resistance pathway in rice similar to the NPR1-mediated signaling pathway in Arabidopsis [J].

Chern, MS ;

Fitzgerald, HA ;

Yadav, RC ;

Canlas, PE ;

Dong, XN ;

Ronald, PC .

PLANT JOURNAL, 2001, 27 (02) :101-113

[7] ARABIDOPSIS SIGNAL-TRANSDUCTION MUTANT DEFECTIVE IN CHEMICALLY AND BIOLOGICALLY INDUCED DISEASE RESISTANCE [J].

DELANEY, TP ;

FRIEDRICH, L ;

RYALS, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (14) :6602-6606

[8] Systemic acquired resistance [J].

Durrant, WE ;

Dong, X .

ANNUAL REVIEW OF PHYTOPATHOLOGY, 2004, 42 :185-209

[9] Elicitor and Fusarium-induced expression of NPR1-like genes in banana [J].

Endah, Rosita ;

Beyene, Getu ;

Kiggundu, Andrew ;

van den Berg, Noelani ;

Schluter, Urte ;

Kunert, Karl ;

Chikwamba, Rachel .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2008, 46 (11) :1007-1014

[10] Overexpression of (At)NPR1 in rice leads to a BTH- and environment-induced lesion-mimic/cell death phenotype [J].

Fitzgerald, HA ;

Chern, MS ;

Navarre, R ;

Ronald, PC .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2004, 17 (02) :140-151

← 1 2 3 4 →