Electrochemical detection of in situ adriamycin oxidative damage to DNA

被引：126

作者：

Oliveira-Brett, AM ^{[1
]}

Vivan, M

Fernandes, IR

Piedade, JAP

机构：

[1] Univ Coimbra, Fac Ciencias & Tecnol, Dept Quim, P-3004535 Coimbra, Portugal

[2] Hosp Univ Coimbra, P-3000075 Coimbra, Portugal

来源：

TALANTA | 2002年 / 56卷 / 05期

关键词：

adriamycin; DNA-biosensor; oxidative damage; 8-oxoguanine; voltammetry;

D O I：

10.1016/S0039-9140(01)00656-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Adriamycin intercalation and in situ interaction with double helix DNA was investigated using a voltammetric DNA-biosensor. Oxidation and reduction of adriamycin molecules intercalated in double helix DNA were investigated in order to understand the in vivo mechanism of action with this anti-neoplasic drug. The results showed that the interaction of adriamycin with DNA is potential-dependent causing contact between DNA guanine and adenine bases and the electrode surface such that their oxidation is easily detected. A mechanism for adriamycin reduction and oxidation in situ when intercalated in double helix DNA immobilised onto the glassy carbon electrode surface is presented and the formation of the mutagenic 8-oxoguanine explained. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：959 / 970

页数：12

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