Electrochemical determination of carboplatin in serum using a DNA-modified glassy carbon electrode

被引：85

作者：

Brett, AMO ^{[1
]}

Serrano, SHP ^{[1
]}

Macedo, TA ^{[1
]}

Raimundo, D ^{[1
]}

Marques, MH ^{[1
]}

LaScalea, MA ^{[1
]}

机构：

[1] UNIV COIMBRA,FAC MED,P-3000 COIMBRA,PORTUGAL

来源：

ELECTROANALYSIS | 1996年 / 8卷 / 11期

关键词：

carboplatin; DNA; anticancer drug; modified electrode;

D O I：

10.1002/elan.1140081104

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical method for the determination of the anticancer platinum drug carboplatin using a DNA-modified electrode was developed. This electrode was successfully used for the electrochemical determination of carboplatin in serum samples of patients with ovarian cancer undergoing treatment. The electrochemical results clearly demonstrated that, for low concentrations, carboplatin interacts preferentially with adenine rather than guanine groups in the DNA and they can contribute to clarifying the mechanisms of interaction of platinum anticancer drugs with DNA. The pharmacokinetics corresponding to the administration of the drug was followed electrochemically and the detection limit in serum samples was 5.7 x 10(-6)M. Due to a similar mechanism of reaction with DNA other platinum anticancer drugs can be determined by this method.

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收藏

页码：992 / 995

页数：4

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