Selective biosensing of L-lysine by a low-temperature flow-injection technique using an immobilized lysine oxidase reactor

被引:12
作者
Chen, RLC
Lee, MH
Matsumoto, K
机构
[1] Dept. of Food Science and Technology, Faculty of Agriculture, Kyushu University 46-09
关键词
lysine; flow injection analysis; lysine oxidase; biosensor; process monitoring;
D O I
10.2116/analsci.12.87
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
An automated flow-injection analytical system, equipped with an immobilized L-lysine-alpha-oxidase reactor (stable for over 6 months) and having a hydrogen peroxide electrode in the manifold, was developed for biosensing L-lysine. A substantial improvement in the selectivity for lysine was achieved by lowering the system temperature to 10 degrees C; the sensor response was linearly dependent on the L-lysine concentration (from tens of mu M to mM order). The analytical results were verified by HPLC using protein hydrolyzates as test solutions comprising various interfering amino acids. By the aid of an in-line filter, the leaching of lysine from L-lysine-enriched fish feed into an aqueous buffer was automatically monitored.
引用
收藏
页码:87 / 90
页数:4
相关论文
共 20 条
[1]   ELECTROPHYSIOLOGICAL DISTINCTIONS BETWEEN TASTE AND SMELL OF AMINO-ACIDS IN CATFISH [J].
CAPRIO, J .
NATURE, 1977, 266 (5605) :850-851
[2]  
Carr P.W., 1980, IMMOBILIZED ENZYMES
[3]   SEQUENTIAL ENZYMATIC MONITORING OF GLUCOSE, ETHANOL AND GLUTAMATE IN BIOREACTOR FERMENTATION BROTH CONTAINING A HIGH-SALT CONCENTRATION BY A MULTICHANNEL FLOW-INJECTION ANALYSIS METHOD [J].
CHEN, RLC ;
MATSUMOTO, K .
ANALYTICA CHIMICA ACTA, 1995, 308 (1-3) :145-151
[4]   CARBODIIMIDE-MEDIATED COVALENT ATTACHMENT OF LYSINE TO WHEAT GLUTEN AND ITS APPARENT DIGESTIBILITY BY PENAEID SHRIMP [J].
FOX, JM ;
LICHAN, E ;
LAWRENCE, AL .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1995, 43 (03) :733-737
[5]  
GAO LY, 1992, J JPN SOC FOOD SCI, V39, P620
[6]   AMINO-ACID NUTRITION OF FISHES - REQUIREMENTS AND SUPPLEMENTATION OF DIETS [J].
KETOLA, HG .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1982, 73 (01) :17-24
[7]  
KUSAKABE H, 1980, J BIOL CHEM, V255, P976
[8]  
Laidler K J, 1979, Methods Enzymol, V63, P234
[9]   DETERMINATION OF L-GLUTAMATE BY AMPEROMETRIC FLOW-INJECTION ANALYSIS USING IMMOBILIZED GLUTAMATE OXIDASE - MANIFOLD FOR SIMULTANEOUS DETECTION OF COMPONENT SIGNAL AND BLANK SIGNAL [J].
MATSUMOTO, K ;
SAKODA, K ;
OSAJIMA, Y .
ANALYTICA CHIMICA ACTA, 1992, 261 (1-2) :155-159
[10]  
MATSUMOTO K, 1994, J FAC AGR KYUSHU U, V38, P207