Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer

被引：286

作者：

Nakagawa, Takayuki ^{[1
,3
]}

Endo, Hiroyuki ^{[1
]}

Yokoyama, Misa ^{[1
]}

Abe, Jiro ^{[3
]}

Tamai, Keiichi ^{[2
]}

Tanaka, Nobuyuki ^{[2
]}

Sato, Ikuro ^{[4
]}

Takahashi, Satomi ^{[3
]}

Kondo, Takashi ^{[5
]}

Satoh, Kennichi ^{[1
]}

机构：

[1] Miyagi Canc Ctr, Res Inst, Div Canc Stem Cell, Natori, Miyagi 9811293, Japan

[2] Miyagi Canc Ctr, Res Inst, Natori, Miyagi 9811293, Japan

[3] Miyagi Canc Ctr, Dept Thorac Surg, Natori, Miyagi 9811293, Japan

[4] Miyagi Canc Ctr, Dept Pathol, Natori, Miyagi 9811293, Japan

[5] Tohoku Univ, Inst Dev Aging & Canc, Dept Thorac Surg, Aoba Ku, Sendai, Miyagi 9808575, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 436卷 / 02期

关键词：

HOTAIR; Non-coding RNA; Non-small cell lung cancer; Invasion; Metastasis; GENE; EXPRESSION; MUTATIONS; GEFITINIB; DIAGNOSIS; SNAIL;

D O I：

10.1016/j.bbrc.2013.05.101

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HOTAIR is one of long non-coding RNAs and its expression correlates with the prognosis and metastasis in various cancers. We showed that HOTAIR expression has an important role in the development of non-small cell lung cancer (NSCLC). In this study, we examined the expression of HOTAIR in 77 NSCLCs, their corresponding normal lung tissues and 6 brain metastases by quantitative real-time RT-PCR. High expression of HOTAIR (tumor/normal ratio >= 2) was detected in 17 patients (22.1%) and was frequently found in patients with advanced stage, lymph node metastasis or lymph-vascular invasion and short disease free interval. Furthermore, brain metastases show significantly higher HOTAIR expression compared to primary cancer tissues. HOTAIR-expressing A549 cells showed induced cell migration and anchorage-independent cell growth in vitro. These results indicate the expression of HOTAIR enhanced the aggressive behavior of NSCLC cells. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：319 / 324

页数：6

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