Enzyme assay for the rapid determination of plasma lysine

被引：6

作者：

Beckett, PR ^{[1
]}

WrayCahen, D ^{[1
]}

Davis, TA ^{[1
]}

Copeland, KC ^{[1
]}

机构：

[1] BAYLOR COLL MED,USDA ARS,CHILDRENS NUTR RES CTR,HOUSTON,TX 77030

来源：

ANALYTICAL COMMUNICATIONS | 1996年 / 33卷 / 02期

关键词：

D O I：

10.1039/ac9963300047

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The enzymic determination of lysine, using saccharopine dehydrogenase, has been modified to determine plasma lysine concentrations in approximately 5 min. The correlation between lysine concentration determined by using HPLC and this enzymic assay was high (r(2) = 0.92). The main interfering compound was leucine which, at 250 mu mol 1(-1), inhibited the rate measured with lysine by 17%. One application of this method allows plasma lysine to be 'clamped' under hyperinsulinemic euglycemic conditions, The technique may be applicable to the bedside investigation of the roles of hormones and nutrition in the regulation of protein metabolism.

引用

页码：47 / 50

页数：4

共 11 条

[1] RAPID HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD TO MEASURE PLASMA LEUCINE - IMPORTANCE IN THE STUDY OF LEUCINE KINETICS INVIVO
BROWN, LL
WILLIAMS, PE
BECKER, TA
ENSLEY, RJ
MAY, ME
ABUMRAD, NN
[J]. JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 426 (02): : 370 - 375
[2] ENHANCED RESPONSE OF MUSCLE PROTEIN-SYNTHESIS AND PLASMA-INSULIN TO FOOD-INTAKE IN SUCKLED RATS
DAVIS, TA
FIOROTTO, ML
NGUYEN, HV
REEDS, PJ
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (02): : R334 - R340
[3] GROWTH AND METABOLISM OF GASTROINTESTINAL AND SKELETAL-MUSCLE TISSUES IN PROTEIN-MALNOURISHED NEONATAL PIGS
EBNER, S
SCHOKNECHT, P
REEDS, P
BURRIN, D
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1994, 266 (06): : R1736 - R1743
[4] AMINO-ACIDS ENHANCE INSULIN RESISTANCE TO EXOGENOUS GLUCOSE-INFUSION IN OVERNIGHT-FASTED HUMANS
FLAKOLL, PJ
KULAYLAT, M
FREXESSTEED, M
HILL, JO
ABUMRAD, NN
[J]. JOURNAL OF PARENTERAL AND ENTERAL NUTRITION, 1991, 15 (02) : 123 - 127
[5] ROLE OF INSULIN AND BRANCHED-CHAIN AMINO-ACIDS IN REGULATING PROTEIN-METABOLISM DURING FASTING
FREXESSTEED, M
WARNER, ML
BULUS, N
FLAKOLL, P
ABUMRAD, NN
[J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 258 (06): : E907 - E917
[6] LEUCINE OXIDATION CHANGES RAPIDLY AFTER DIETARY-PROTEIN INTAKE IS ALTERED IN ADULT WOMEN BUT LYSINE FLUX IS UNCHANGED AS IS LYSINE INCORPORATION INTO VLDL-APOLIPOPROTEIN B-100
MOTIL, KJ
OPEKUN, AR
MONTANDON, CM
BERTHOLD, HK
DAVIS, TA
KLEIN, PD
REEDS, PJ
[J]. JOURNAL OF NUTRITION, 1994, 124 (01) : 41 - 51
[7] ENZYMIC DETERMINATION OF L-LYSINE IN BIOLOGICAL-MATERIALS
NAKATANI, Y
HIGASHINO, K
FUJIOKA, M
[J]. ANALYTICAL BIOCHEMISTRY, 1972, 49 (01) : 225 - +
[8] ENZYME ELECTRODE FOR SPECIFIC DETERMINATION OF L-LYSINE
ROMETTE, JL
YANG, JS
KUSAKABE, H
[J]. BIOTECHNOLOGY AND BIOENGINEERING, 1983, 25 (11) : 2557 - 2566
[9] USE OF A LEUCINE CLAMP TO DEMONSTRATE THAT IGF-I ACTIVELY STIMULATES PROTEIN-SYNTHESIS IN NORMAL HUMANS
RUSSELLJONES, DL
UMPLEBY, AM
HENNESSY, TR
BOWES, SB
SHOJAEEMORADIE, F
HOPKINS, KD
JACKSON, NC
KELLY, JM
JONES, RH
SONKSEN, PH
[J]. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1994, 267 (04): : E591 - E598
[10] CHANGES IN CEREBROSPINAL-FLUID AND PLASMA AMINO-ACID-CONCENTRATIONS WITH ELEVATED DIETARY-PROTEIN CONCENTRATION IN DOGS WITH PORTACAVAL SHUNTS
SCHAEFFER, MC
ROGERS, QR
LEUNG, PMB
WOLFE, BM
STROMBECK, DR
[J]. LIFE SCIENCES, 1991, 48 (23) : 2215 - 2223

← 1 2 →