Sulfated modification of the polysaccharide from Cordyceps_gunnii mycelia and its biological activities

被引：82

作者：

Zhu, Zhen-Yuan ^{[1
]}

Liu, Yang ^{[1
]}

Si, Chuan-Ling ^{[2
]}

Yuan, Jing ^{[1
]}

Lv, Qiang ^{[1
]}

Li, Yuan-Yuan ^{[1
]}

Dong, Guo-Ling ^{[1
]}

Liu, An-Jun ^{[1
]}

Zhang, Yong-Min ^{[3
]}

机构：

[1] Tianjin Univ Sci & Technol, Coll Food Sci & Biotechnol, Minist Educ, Key Lab Food Nutr & Safety, Tianjin 300457, Peoples R China

[2] Tianjin Univ Sci & Technol, Coll Mat Sci & Chem Engn, Tianjin Key Lab Pulp & Paper, Tianjin 300457, Peoples R China

[3] Univ Paris 06, CNRS, Inst Parisien Chim Mol, UMR 7201, F-75005 Paris, France

来源：

CARBOHYDRATE POLYMERS | 2013年 / 92卷 / 01期

基金：

中国国家自然科学基金; 国家高技术研究发展计划(863计划);

关键词：

Sulfated modification; Polysaccharide; Cordyceps_gunnii; Anti-tumor activity; Anti-oxidant activity; HIGH-MOLECULAR-WEIGHT; ANTITUMOR-ACTIVITY; ANTICOAGULANT; DERIVATIVES;

D O I：

10.1016/j.carbpol.2012.10.007

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

A chemically new sulfated polysaccharide (SPS50) was prepared from the water soluble polysaccharide (PS50), isolated from Cordyceps_gunnii mycelia, by concentrated sulfuric acid method. The yield of crude SPS50 was 62.34% and its specific rotation was [alpha](D)(20) = 36.75 degrees. The structural characteristics of this chemically sulfated polysaccharide were determined based on the infrared analysis (IR), high performance liquid chromatography (HPLC) and sodium dodecyl sulfate polyacrylamide gel electropheresis (SDS-PAGE). Its biological properties including anti-oxidant and anti-tumor activities were also investigated. The results showed that the anti-oxidant capacity of SPS50 was not as good as SP50 and the anti-tumor activity of SPS50 was much better than PS50. SPS50 showed evident growth inhibition on K562 cells. The tumor inhibition ratio of SPS50 against K562 cells was 69.92%. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：872 / 876

页数：5

共 22 条

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