Characterization and antitumor activity of a polysaccharide from Strongylocentrotus nudus eggs

被引：152

作者：

Liu, Chunhui ^{[1
]}

Lin, Qinxiong ^{[1
]}

Gao, Yi ^{[1
]}

Ye, Liang ^{[1
]}

Xing, Yingying ^{[1
]}

Xi, Tao ^{[1
]}

机构：

[1] China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2007年 / 67卷 / 03期

关键词：

sea urchin; Strongylocentrotus nudus; eggs; D-glucan; structure; antitumor;

D O I：

10.1016/j.carbpol.2006.05.024

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A water-soluble polysaccharide named as SEP was isolated from the eggs of Dalian purple sea urchin Strongylocentrotus nudus by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its antitumor activity. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, GLC-MS, methylation analysis, Periodate oxidation and Smith degradation. Based on the data obtained, SEP was found to be an alpha-(1 -> 4)-D-glucan, with a single alpha-D-glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 1.95 x 10(6) Da. Pharmacological studies revealed SEP could inhibit the growth of Sarcoma 180 tumor remarkably in vivo. SEP also stimulated the spleen lymphocyte proliferation in S180-bearing mice, indicating its antitumor effect may be related to immunomodulation. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：313 / 318

页数：6

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