Application of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) to investigate trace metal spatial distributions in human tooth enamel and dentine growth layers and pulp

被引：104

作者：

Kang, D ^{[1
]}

Amarasiriwardena, D ^{[1
]}

Goodman, AH ^{[1
]}

机构：

[1] Hampshire Coll, Sch Nat Sci, Amherst, MA 01002 USA

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2004年 / 378卷 / 06期

基金：

美国国家科学基金会;

关键词：

human teeth; trace metals; enamel growth layers; micro spatial distribution; laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS);

D O I：

10.1007/s00216-004-2504-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human tooth enamel provides a nearly permanent and chronological record of an individual's nutritional status and anthropogenic trace metal exposure during development; it might thus provide an excellent bio archive. We investigated the micro-spatial distribution of trace metals (Cu, Fe, Mg, Sr, Pb, and Zn) in 196x339 mum(2) raster pattern areas (6.6x10(4) mum(2)) in a deciduous tooth using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). Ablated areas include prenatal and postnatal enamel, the neonatal line, the dentine-enamel junction (DEJ), dentine, and the dentine-pulp junction. Topographic variations in the surface elemental distribution of lead, zinc, strontium, and iron intensities in a deciduous tooth revealed heterogeneous distribution within and among regions. Ca-43 normalized elemental intensities showed the following order: Sr>Mg>>Zn>Pb>Fe>Cu. Elevated zinc and lead levels were present in the dental pulp region and at the neonatal line. This study demonstrates the ability of LA-ICP-MS to provide unique elemental distribution information in micro spatial areas of dental hard tissues. Elemental distribution plots could be useful in decoding nutrition and pollution information embedded in their bio apatite structure.

引用

页码：1608 / 1615

页数：8

共 27 条

[11] THE ANALYSIS AND LEVELS OF LEAD IN HUMAN-TEETH - A REVIEW [J].