Quantitative phase-contrast microscopy by a lateral shear approach to digital holographic image reconstruction

被引：145

作者：

Ferraro, P.

Alferi, D.

De Nicola, S.

De Petrocellis, L.

Finizio, A.

Pierattini, G.

机构：

[1] CNR, Ist Nazl Ottica Applicata, I-80072 Pozzuoli, NA, Italy

[2] CNR, Ist Cibernet E Caianiello, I-80072 Pozzuoli, NA, Italy

来源：

OPTICS LETTERS | 2006年 / 31卷 / 10期

关键词：

D O I：

10.1364/OL.31.001405

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Combining the concept of lateral shear interferometry (LST) within a digital holography microscope, we demonstrate that it is possible to obtain quantitative optical phase measurement in microscopy by a new single-image-processing procedure. Numerical lateral shear of the reconstructed wavefront in the image plane makes it possible to retrieve the derivative of the wavefront and remove the defocus aberration term introduced by the microscope objective. The method is tested to investigate a silicon structure and a mouse cell line. (c) 2006 Optical Society of America

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收藏

页码：1405 / 1407

页数：3

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