Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms

被引：954

作者：

Cuche, E ^{[1
]}

Marquet, P

Depeursinge, C

机构：

[1] Swiss Fed Inst Technol, Inst Appl Opt, CH-1015 Lausanne, Switzerland

[2] Univ Lausanne, Dept Neurol, Lab Neurol Res, Inst Physiol, CH-1005 Lausanne, Switzerland

来源：

APPLIED OPTICS | 1999年 / 38卷 / 34期

关键词：

D O I：

10.1364/AO.38.006994

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present a digital method for holographic microscopy involving a CCD camera as a recording device. Off-axis holograms recorded with a magnified image of microscopic objects are numerically reconstructed in amplitude and phase by calculation of scaler diffraction in the Fresnel approximation. For phase-contrast imaging the reconstruction method involves the computation of a digital replica of the reference wave. A digital method for the correction of the phase aberrations is presented. We present a detailed description of the reconstruction procedure and show that the transverse resolution is equal to the diffraction limit of the imaging system. (C) 1999 Optical Society of America OCIS codes: 090.0090, 110.0180, 040.1520, 350.5030, 090.1000.

引用

页码：6994 / 7001

页数：8

共 20 条

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