Engineering chromosomes for delivery of therapeutic genes

被引：17

作者：

Irvine, DV ^{[1
]}

Shaw, ML ^{[1
]}

Choo, KHA ^{[1
]}

Saffery, R ^{[1
]}

机构：

[1] Univ Melbourne, Chromosome Res Grp, Murdoch Childrens Res Inst, Royal Childrens Hosp,Dept Paediat, Parkville, Vic 3052, Australia

来源：

TRENDS IN BIOTECHNOLOGY | 2005年 / 23卷 / 12期

关键词：

D O I：

10.1016/j.tibtech.2005.10.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The ability to create fully functional human chromosome vectors represents a potentially exciting gene-delivery system for the correction of human genetic disorders with several advantages over viral delivery systems. However, for the full potential of chromosome-based gene-delivery vectors to be realized, several key obstacles must be overcome. Methods must be developed to insert therapeutic genes reliably and efficiently and to enable the stable transfer of the resulting chromosomal vectors to different therapeutic cell types. Research to achieve these outcomes continues to encounter major challenges; however recent developments have reiterated the potential of chromosome-based vectors for therapeutic gene delivery. Here we review the different strategies under development and discuss the advantages and problems associated with each.

引用

页码：575 / 583

页数：9

共 90 条

[51] A mammalian artificial chromosome engineering system (ACE System) applicable to biopharmaceutical protein production, transgenesis and gene-based cell therapy [J].