Identification of candidate genes for phenolics accumulation in tomato fruit

被引：42

作者：

Di Matteo, Antonio ^{[1
]}

Ruggieri, Valentino ^{[1
]}

Sacco, Adriana ^{[1
]}

Rigano, Maria Manuela ^{[1
]}

Carriero, Filomena ^{[2
]}

Bolger, Anthony ^{[3
,4
]}

Fernie, Alisdair R. ^{[3
]}

Frusciante, Luigi ^{[1
]}

Barone, Amalia ^{[1
]}

机构：

[1] Univ Naples Federico II, Dept Agr Sci, I-80055 Portici, Italy

[2] Metapontum Agrobios, I-75010 Metaponto, MT, Italy

[3] Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany

[4] Rhein Westfal TH Aachen, D-52062 Aachen, Germany

来源：

PLANT SCIENCE | 2013年 / 205卷

关键词：

Solanum lycopersicum; Flavonoids; Introgression lines; Microarray; TILLING; Ethylene responsive factor 1; ANTHOCYANIC VACUOLAR INCLUSIONS; LYCOPERSICON-PENNELLII; QTL ANALYSIS; EXPRESSION; TRANSPORT; ETHYLENE; ARABIDOPSIS; SEQUESTRATION; ANTIOXIDANTS; TRAFFICKING;

D O I：

10.1016/j.plantsci.2013.02.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phenolics are antioxidants present in tomato fruit that confer healthy benefits and exhibit crucial roles for plant metabolism and response to environmental stimuli. An approach based on two genomics platforms was undertaken to identify candidate genes associated to higher phenolics content in tomato fruit. A comparative transcriptomic analysis between the S. pennellii Introgression Line 7-3, which produced an average higher level of fruit phenolics, and the cultivated variety M82, revealed that their differences are attributed to genes involved in phenolics accumulation into the vacuole. The up-regulation of genes coding for one MATE-transporter, one vacuolar sorting protein and three GSTs supported this hypothesis. The observed balancing effect between two ethylene responsive factors (ERF1 and ERF4) was also hypothesized to drive the transcriptional regulation of these transport genes. In order to confirm such model a TILLING platform was explored. A mutant was isolated harbouring a point mutation in the ERF1 cds that affects the protein sequence and its expected function. Fruits of the mutant exhibited a significant reduced level of phenolics than the control variety. Changes in the expression of genes involved in sequestration of phenolics in vacuole also supported the hypothesized key-role of ERF1 in orchestrating these genes. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：87 / 96

页数：10

共 59 条

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