Resolution improvement from 'in situ annealing' of copper nitrite reductase crystals

被引:15
作者
Ellis, MJ
Antonyuk, S
Hasnain, SS [1 ]
机构
[1] De Montfort Univ, Fac Sci Appl, Leicester LE1 9BH, Leics, England
[2] SERC, Daresbury Lab, CLRC, Mol Biophys Grp, Warrington WA4 4AD, Cheshire, England
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷
关键词
D O I
10.1107/S0907444902000136
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Significant improvement of the resolution of copper nitrite reductase crystals was achieved by using the in situ annealing technique. The effective resolution limits increased by 1.5 Angstrom from 2.5 to 1.0 Angstrom, the mosaicity value decreased from 1.5 to 0.3degrees and the spot shape changed from elliptical to circular.
引用
收藏
页码:456 / 458
页数:3
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