Caveolin-2 localizes to the Golgi complex but redistributes to plasma membrane, caveolae, and rafts when co-expressed with caveolin-1

被引:177
作者
Mora, R
Bonilha, VL
Marmorstein, A
Scherer, PE
Brown, D
Lisanti, MP
Rodriguez-Boulan, E
机构
[1] Cornell Univ, Weill Med Coll, Dyson Vis Res Inst, Dept Ophthalmol, New York, NY 10021 USA
[2] Cornell Univ, Weill Med Coll, Dept Cell Biol, New York, NY 10021 USA
[3] Massachusetts Gen Hosp, Renal Unit, Boston, MA 02129 USA
[4] Massachusetts Gen Hosp, Dept Pathol, Boston, MA 02129 USA
[5] Harvard Univ, Sch Med, Boston, MA 02129 USA
[6] Yeshiva Univ Albert Einstein Coll Med, Dept Mol Pharmacol, Bronx, NY 10461 USA
[7] Yeshiva Univ Albert Einstein Coll Med, Dept Cell Biol, Bronx, NY 10461 USA
关键词
D O I
10.1074/jbc.274.36.25708
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have characterized comparatively the subcellular distributions of caveolins-1 and -2, their interactions and their roles in caveolar formation in polarized epithelial cells. In Fischer rat thyroid (FRT) cells, which express low levels of caveolin-2 and no caveolin-1, caveolin-2 localizes exclusively to the Golgi complex but is partially redistributed to the plasma membrane upon co-expression of caveolin-1 by transfection or by adenovirus-mediated transduction. In Madin-Darby canine kidney (MDCK) cells, which constitutively express both caveolin-1 and -2, caveolin-2 localized to both the Gels complex and to the plasma membrane, where it co-distributed with caveolin-1 in flat patches and in caveolae. In FRT cells, endogenous or overexpressed caveolin-2 did not associate with low density Triton insoluble membranes that floated in sucrose density gradients but was recruited to these membranes when co-expressed together with caveolin-1. In MDCK cells, both caveolin-1 and caveolin-2 associated with low density Triton-insoluble membranes. In FRT cells, transfection of caveolin-1 promoted the assembly of plasma membrane caveolae that localized preferentially (over 99%) to the basolateral surface, like constitutive caveolae of MDCK cells. In contrast, as expected from its intracellular distribution, endogenous or overexpressed caveolin-2 did not promote the assembly of caveolae; rather, it appeared to promote the assembly of intracellular vesicles in the peri-Golgi area. The data reported here demonstrate that caveolin-1 and -2 have different and complementary subcellular localizations and functional properties in polarized epithelial cells and suggest that the two proteins co-operate to carry out specific as yet unknown tasks between the Golgi complex and the cell surface.
引用
收藏
页码:25708 / 25717
页数:10
相关论文
共 31 条
  • [11] Caveolin transfection results in caveolae formation but not apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins in epithelial cells
    Lipardi, C
    Mora, R
    Colomer, V
    Paladino, S
    Nitsch, L
    Rodriguez-Boulan, E
    Zurzolo, C
    [J]. JOURNAL OF CELL BIOLOGY, 1998, 140 (03) : 617 - 626
  • [12] ULTRASTRUCTURAL EVIDENCE OF DIFFERENTIAL SOLUBILITY IN TRITON X-100 OF ENDOTHELIAL VESICLES AND PLASMA-MEMBRANE
    MOLDOVAN, NI
    HELTIANU, C
    SIMIONESCU, N
    SIMIONESCU, M
    [J]. EXPERIMENTAL CELL RESEARCH, 1995, 219 (01) : 309 - 313
  • [13] Oligomerization of VIP21-caveolin in vitro is stabilized by long chain fatty acylation or cholesterol
    Monier, S
    Dietzen, DJ
    Hastings, WR
    Lublin, DM
    Kurzchalia, TV
    [J]. FEBS LETTERS, 1996, 388 (2-3) : 143 - 149
  • [14] VIP21/CAVEOLIN IS A CHOLESTEROL-BINDING PROTEIN
    MURATA, M
    PERANEN, J
    SCHREINER, R
    WIELAND, F
    KURZCHALIA, TV
    SIMONS, K
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (22) : 10339 - 10343
  • [15] Caveolins, a family of scaffolding proteins for organizing "preassembled signaling complexes" at the plasma membrane"
    Okamoto, T
    Schlegel, A
    Scherer, PE
    Lisanti, MP
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (10) : 5419 - 5422
  • [16] STRUCTURAL MODULATIONS OF PLASMALEMMAL VESICLES
    PALADE, GE
    BRUNS, RR
    [J]. JOURNAL OF CELL BIOLOGY, 1968, 37 (03) : 633 - +
  • [17] PALADE GE, 1953, J APPL PHYS, V24, P1424
  • [18] Caveolae and caveolins
    Parton, RG
    [J]. CURRENT OPINION IN CELL BIOLOGY, 1996, 8 (04) : 542 - 548
  • [19] REGULATED INTERNALIZATION OF CAVEOLAE
    PARTON, RG
    JOGGERST, B
    SIMONS, K
    [J]. JOURNAL OF CELL BIOLOGY, 1994, 127 (05) : 1199 - 1215
  • [20] MORPHOGENESIS OF THE POLARIZED EPITHELIAL-CELL PHENOTYPE
    RODRIGUEZBOULAN, E
    NELSON, WJ
    [J]. SCIENCE, 1989, 245 (4919) : 718 - 725