Environmental genome shotgun sequencing of the Sargasso Sea

被引：2843

作者：

Venter, JC

Remington, K

Heidelberg, JF

Halpern, AL

Rusch, D

Eisen, JA

Wu, DY

Paulsen, I

Nelson, KE

Nelson, W

Fouts, DE

Levy, S

Knap, AH

Lomas, MW

Nealson, K

White, O

Peterson, J

Hoffman, J

Parsons, R

Baden-Tillson, H

Pfannkoch, C

Rogers, YH

Smith, HO

机构：

[1] Inst Biol Energy Alternat, Rockville, MD 20850 USA

[2] Ctr Advancement Genom, Rockville, MD 20850 USA

[3] Inst Genom Res, Rockville, MD 20850 USA

[4] J Craig Venter Sci Fdn, Joint Technol Ctr, Rockville, MD 20850 USA

[5] Univ So Calif, Los Angeles, CA 90089 USA

[6] Bermuda Biol Stn Res Inc, St George GE01, Bermuda

来源：

SCIENCE | 2004年 / 304卷 / 5667期

关键词：

D O I：

10.1126/science.1093857

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have applied "whole-genome shotgun sequencing" to microbial populations collected en masse on tangential flow and impact filters from seawater samples collected from the Sargasso Sea near Bermuda. A total of 1.045 billion base pairs of nonredundant sequence was generated, annotated, and analyzed to elucidate the gene content, diversity, and relative abundance of the organisms within these environmental samples. These data are estimated to derive from at least 1800 genomic species based on sequence relatedness, including 148 previously unknown bacterial phylotypes. We have identified over 1.2 million previously unknown genes represented in these samples, including more than 782 new rhodopsin-like photoreceptors. Variation in species present and stoichiometry suggests substantial oceanic microbial diversity.

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收藏

页码：66 / 74

页数：9

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