Genetic tools for pseudomonads, rhizobia, and other gram-negative bacteria

被引：26

作者：

Davison, J ^{[1
]}

机构：

[1] INRA, Lab Biol Cellulaire, F-78260 Versailles, France

来源：

BIOTECHNIQUES | 2002年 / 32卷 / 02期

关键词：

D O I：

10.2144/02322rv02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gram-negative bacteria are extraordinarily diverse microorganisms that present a wide variety of characteristics worthy of genetic investigation. For historical reasons, the application of recombinant DIVA technology to Gram-negative bacteria in general has always lagged behind that of E. coli and its close relatives. However the past 10 years have seen dramatic advances in the development of new tools and vectors for genetic analysis in non-E. coli hosts. Applications include various kinds of genetic manipulation, conjugation, transposition, site-specific recombination, protein secretion, protein, purification cell suicide, microbial ecology, biodegradation, and plant and animal pathogenicity.

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页码：386 / +

页数：12

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