Effects of low temperatures on proliferation-related signaling pathways in the hippocampus after traumatic brain injury

被引：26

作者：

Wang, Yang ^{[1
,2
]}

Guo, Fei ^{[2
]}

Pan, Changfu ^{[3
]}

Lou, Yuanlei ^{[2
]}

Zhang, Pengqi ^{[4
]}

Guo, Shangchun ^{[1
]}

Yin, Junhui ^{[1
]}

Deng, Zhifeng ^{[3
,4
]}

机构：

[1] Shanghai Jiao Tong Univ, Affiliated Peoples Hosp 6, Inst Orthopaed Surg, Shanghai 200233, Peoples R China

[2] Nanchang Univ, Inst Urol, Nanchang 330006, Peoples R China

[3] Nanchang Univ, Affiliated Hosp 3, Dept Neurosurg, Nanchang 330006, Peoples R China

[4] Shanghai Jiao Tong Univ, Dept Neurosurg, Affiliated Peoples Hosp 6, Shanghai 200233, Peoples R China

来源：

EXPERIMENTAL BIOLOGY AND MEDICINE | 2012年 / 237卷 / 12期

基金：

中国国家自然科学基金;

关键词：

miRNA; microarray; neurogenesis; traumatic brain injury; low-temperature exposure; CLOSED-HEAD INJURY; MICRORNA EXPRESSION; RAT; NEUROGENESIS; NEURONS; CELLS; HYPOTHERMIA; NOTCH; MICE;

D O I：

10.1258/ebm.2012.012123

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

To evaluate the influence of low temperatures on the proliferation of neural stem cells (NSCs) and the regulation of their signaling pathways after brain trauma, we examined changes in the expression levels of specific miRNAs and their target genes. We also evaluated NSC proliferation in the hippocampus after brain trauma under low-temperature conditions. We found that the expression profile of miRNAs in the hippocampus after trauma changed at both normal and low temperatures, and the expression of miR-34a decreased significantly lower in rats exposed to low temperatures. There was significant proliferation of endogenous NSCs in the hippocampus after brain trauma at both temperatures, but NSC proliferation was slower at low temperatures. In addition, the expression of Notch1 significantly increased in the hippocampus after brain trauma at both temperatures. However, at low temperatures, the degree of up-regulation of Notch signaling molecules was significantly lower. We conclude that low-temperature environments can inhibit the proliferation of endogenous NSCs in the hippocampus, possibly by alleviating the effects of miR-34a down-regulation and Notch signaling up-regulation induced by traumatic brain injury.

引用

页码：1424 / 1432

页数：9

共 21 条

[1]

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