Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

被引：366

作者：

Argyros, Rebecca D. ^{[1
]}

Mathews, Dennis E. ^{[2
]}

Chiang, Yi-Hsuan ^{[1
]}

Palmer, Christine M. ^{[1
]}

Thibault, Derek M. ^{[1
]}

Etheridge, Naomi ^{[1
]}

Argyros, D. Aaron ^{[1
]}

Mason, Michael G. ^{[1
]}

Kieber, Joseph J. ^{[3
]}

Schaller, G. Eric ^{[1
]}

机构：

[1] Dartmouth Coll, Dept Biol Sci, Hanover, NH 03755 USA

[2] Univ New Hampshire, Dept Plant Biol, Durham, NH 03824 USA

[3] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA

来源：

PLANT CELL | 2008年 / 20卷 / 08期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1105/tpc.108.059584

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The type B Arabidopsis Response Regulators (ARRs) of Arabidopsis thaliana are transcription factors that act as positive regulators in the two-component cytokinin signaling pathway. We employed a mutant-based approach to perform a detailed characterization of the roles of ARR1, ARR10, and ARR12 in plant growth and development. The most pronounced phenotype was found in the arr1-3 arr10-5 arr12-1 triple loss-of-function mutant, which showed almost complete insensitivity to high levels of exogenously applied cytokinins. The triple mutant exhibited reduced stature due to decreased cell division in the shoot, enhanced seed size, increased sensitivity to light, altered chlorophyll and anthocyanin concentrations, and an aborted primary root with protoxylem but no metaxylem. Microarray analysis revealed that expression of the majority of cytokinin-regulated genes requires the function of ARR1, ARR10, and ARR12. Characterization of double mutants revealed differing contributions of the type B ARRs to mutant phenotypes. Our results support a model in which cytokinin regulates a wide array of downstream responses through the action of a multistep phosphorelay that culminates in transcriptional regulation by ARR1, ARR10, and ARR12.

引用

页码：2102 / 2116

页数：15

共 60 条

[11] Cytokinins. New insights into a classic phytohormone [J].

Haberer, G ;

Kieber, JJ .

PLANT PHYSIOLOGY, 2002, 128 (02) :354-362

[12] Cytokinin signal perception and transduction [J].

Heyl, A ;

Schmülling, T .

CURRENT OPINION IN PLANT BIOLOGY, 2003, 6 (05) :480-488

[13] In planta functions of the Arabidopsis cytokinin receptor family [J].

Higuchi, M ;

Pischke, MS ;

Mähönen, AP ;

Miyawaki, K ;

Hashimoto, Y ;

Seki, M ;

Kobayashi, M ;

Shinozaki, K ;

Kato, T ;

Tabata, S ;

Helariutta, Y ;

Sussman, MR ;

Kakimoto, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (23) :8821-8826

[14] Molecular structure of the GARP family of plant Myb-related DNA binding motifs of the Arabidopsis response regulators [J].

Hosoda, K ;

Imamura, A ;

Katoh, E ;

Hatta, T ;

Tachiki, M ;

Yamada, H ;

Mizuno, T ;

Yamazaki, T .

PLANT CELL, 2002, 14 (09) :2015-2029

[15] Monitoring genome-wide changes in gene expression in response to endogenous cytokinin reveals targets in Arabidopsis thaliana [J].

Hoth, S ;

Ikeda, Y ;

Morgante, M ;

Wang, XJ ;

Zuo, JR ;

Hanafey, MK ;

Gaasterland, T ;

Tingey, SV ;

Chua, NH .

FEBS LETTERS, 2003, 554 (03) :373-380

[16] The Arabidopsis histidine phosphotransfer proteins are redundant positive regulators of cytokinin signaling [J].

Hutchison, Claire E. ;

Li, Jie ;

Argueso, Cristiana ;

Gonzalez, Monica ;

Lee, Eurie ;

Lewis, Michael W. ;

Maxwell, Bridey B. ;

Perdue, Tony D. ;

Schaller, G. Eric ;

Alonso, Jose M. ;

Ecker, Joseph R. ;

Kieber, Joseph J. .

PLANT CELL, 2006, 18 (11) :3073-3087

[17] Two-component circuitry in Arabidopsis cytokinin signal transduction [J].

Hwang, I ;

Sheen, J .

NATURE, 2001, 413 (6854) :383-389

[18] Compilation and characterization of Arabidopsis thaliana response regulators implicated in His-Asp phosphorelay signal transduction [J].

Imamura, A ;

Hanaki, N ;

Nakamura, A ;

Suzuki, T ;

Taniguchi, M ;

Kiba, T ;

Ueguchi, C ;

Sugiyama, T ;

Mizuno, T .

PLANT AND CELL PHYSIOLOGY, 1999, 40 (07) :733-742

[19] In vivo and in vitro characterization of the ARR11 response regulator implicated in the His-to-Asp phosphorelay signal transduction in Arabidopsis thaliana [J].

Imamura, A ;

Kiba, T ;

Tajima, Y ;

Yamashino, T ;

Mizuno, T .

PLANT AND CELL PHYSIOLOGY, 2003, 44 (02) :122-131

[20] Cellular localization of the signaling components of Arabidopsis His-to-Asp phosphorelay [J].

Imamura, A ;

Yoshino, Y ;

Mizuno, T .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2001, 65 (09) :2113-2117

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