Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and co-localization with the SpoOJ partitioning protein

被引：162

作者：

Lewis, PJ

Errington, J

机构：

[1] Sir William Dunn School of Pathology, University of Oxford, Oxford, OX1 3RE, South Parks Road

来源：

MOLECULAR MICROBIOLOGY | 1997年 / 25卷 / 05期

关键词：

D O I：

10.1111/j.1365-2958.1997.mmi530.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed methods for labelling regions of the Bacillus subtilis chromosome with the nucleotide analogue 5-bromodeoxyuridine (BrdU) and for subcellular visualization of the labelled DNA. Examination of oriC-labelled chromosomes in outgrowing spores has provided direct evidence for active segregation of sister chromosomes. Co-immunodetection of Spo0J and BrdU-labelled DNA has directly confirmed the expected close association between this chromosome partitioning protein and the oriC region of the chromosome. The results provide further support for the notion that bacterial cells use an active mitotic-like mechanism to segregate their chromosomes.

引用

页码：945 / 954

页数：10

共 32 条

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