Inositides in the nucleus:: presence and characterisation of the isozymes of phospholipase β family in NIH 3T3 cells

被引：38

作者：

Cocco, L ^{[1
]}

Rubbini, S

Manzoli, L

Billi, AM

Faenza, I

Peruzzi, D

Matteucci, A

Artico, M

Gilmour, RS

Rhee, SG

机构：

[1] Univ Bologna, Inst Human Anat, I-40126 Bologna, Italy

[2] Ist Ortoped Rizzoli, CNR, Cytomorphol Inst, I-40136 Bologna, Italy

[3] Ist Ortoped Rizzoli, CNR, Lab Cell Biol & Electron Microscopy, I-40136 Bologna, Italy

[4] Univ Auckland, Sch Med, Dept Mol Med, Auckland, New Zealand

[5] NHLBI, Lab Cell Signalling, NIH, Bethesda, MD 20892 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS | 1999年 / 1438卷 / 02期

关键词：

inositide; nucleus; phospholipase C; isozyme; phosphatidylinositol 4,5-bisphosphate;

D O I：

10.1016/S1388-1981(99)00061-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous reports from our laboratories and others have hinted that the nucleus is a site for an autonomous signalling system acting through the activation of the inositol lipid cycle. Among phospholipases (PLC) it has been shown previously that PLC beta(1) is specifically localised in the nucleus as well as at the plasma membrane. Using NIH 3T3 cells, it has been possible to obtain, with two purification strategies, in the presence of in the absence of Nonidet P-40, both intact nuclei still maintaining the outer membrane and nuclei completely stripped of their envelope. In these nuclei, we show that not only PLC beta(1) is present, but also PLC beta(2), PLC beta(3) and PLC beta(4). The more abounding isoform is PLC beta(1) followed by PLC beta(3), PLC beta(2) and PLC beta(4), respectively. All the isoforms are enriched in nuclear preparations free from nuclear envelope and cytoplasmatic debris, indicating that the actual localisation of the PLC beta isozymes is in the inner nuclear compartment. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：295 / 299

页数：5

共 20 条

[1] Localization of two forms of phospholipase C-β1, a and b, in C6Bu-1 cells [J].