Control of expression of PLCβ1 by LAC repressor system:: Relationship between nuclear PLCβ1 overexpression and growth factor stimulation

Swiss 3T3 cells have a nuclear phosphoinositide signalling system which is under the control of insulin like growth factor I (IGF-I) and acts separately from that at the plasma membrane. By using the Lac repressor system we were able both to obtain the inducible overexpression of phospholipase C beta(1) (PLC beta(1)) and to determine its subcellular localisation and partitioning. Moreover, by comparing the level of expression at the nucleus and the percentage of cells actively incorporating bromodeoxyuridine (BrdU) in S phase it has strengthened the issue of the importance of this PLC in the onset of DNA synthesis mediated by IGF-I. In addition, this system appears to be a very powerful tool for further analysis of the downstream events following the activation of nuclear PLC beta(1). (C) 1997 Academic Press.