Purification and ligand binding of EmrR, a regulator of a multidrug transporter

被引：66

作者：

Brooun, A ^{[1
]}

Tomashek, JJ ^{[1
]}

Lewis, K ^{[1
]}

机构：

[1] Tufts Univ, Ctr Biotechnol, Medford, MA 02155 USA

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 16期

关键词：

D O I：

10.1128/JB.181.16.5131-5133.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

EmrR, the repressor of the emrRAB operon of Escherichia coli, was purified to 95% homogeneity. EmrR was found to bind putative ligands of the EmrAB pump-2,4-dinitrophenol, carbonyl cyanide m-chlorophenylhydrazone, and carbonyl cyanide p- (trifluoro-methoxy) phenylhydrazone-with affinities in the micromolar range. Equilibrium dialysis experiments suggested one bound ligand per monomer of the dimeric EmrR.

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页码：5131 / 5133

页数：3

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