Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773

被引：43

作者：

Kudo, F ^{[1
]}

Yamamoto, Y ^{[1
]}

Yokoyama, K ^{[1
]}

Eguchi, T ^{[1
]}

Kakinuma, K ^{[1
]}

机构：

[1] Tokyo Inst Technol, Dept Chem, Meguro Ku, Tokyo 1528551, Japan

来源：

JOURNAL OF ANTIBIOTICS | 2005年 / 58卷 / 12期

关键词：

2-deoxy-scyllo-inosose synthase; L-glutamine : 2-deoxy-scyllo-inosose aminotransferase; 2-deoxyscyllo-inosamine dehydrogenase; 2-deoxystreptamine; neomycin biosynthetic gene cluster;

D O I：

10.1038/ja.2005.104

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

NeoA, B, and C encoded in the neomycin biosynthetic gene cluster have been enzymatically confirmed to be responsible to the formation of 2deoxystreptamine (DOS) in Streptomyces fradiae. NeoC was functionally characterized as 2-deoxy-scyllo-inosose synthase, which catalyzes the carbocycle formation from D-glucose-6-phosphate to 2-deoxy-scyllo-inosose. Further, NeoA appeared to catalyze the oxidation of 2-deoxy-scyllo-inosamine (DOIA) with NAD(P)(+) forming 3-amino-2,3-dideoxy-scyllo-inosose (amino-DOI). Consequently, NeoA was characterized as 2-deoxy-scyllo-inosamine dehydrogenase. Finally, amino-DOI produced by NeoA from DOIA was transformed into DOS by NeoB. Since NeoB (Neo6) was also reported to be L-glutamine:2deoxy-scyllo-inosose aminotransferase, all the enzymes in the DOS biosynthesis were characterized for the first time.

引用

页码：766 / 774

页数：9

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