A putative twin-arginine translocation pathway in Legionella pneumophila

被引：38

作者：

De Buck, E ^{[1
]}

Lebeau, I ^{[1
]}

Maes, L ^{[1
]}

Geukens, N ^{[1
]}

Meyen, E ^{[1
]}

Van Mellaert, L ^{[1
]}

Anné, J ^{[1
]}

Lammertyn, E ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Bacteriol Lab, Rega Inst Med Res, B-3000 Louvain, Belgium

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 317卷 / 02期

关键词：

tat secretion; tat substrates; expression tat genes; Legionella pneumophila;

D O I：

10.1016/j.bbrc.2004.03.091

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Legionella pneumophila is a facultative intracellular human pathogen causing Legionnaires' disease, a severe form of pneumonia. Because of the importance of secretion pathways in virulence, we were interested in the possible presence of the twin-arginine translocation (Tat) pathway in L. pneumophila. This secretion pathway is used to transport folded proteins, characterized by two arginines in their signal peptide, across the cytoplasmic membrane. We describe here the presence of a putative Tat pathway in L. pneumophila. Three genes encoding Escherichia coli TatA, TatB, and TatC homologues were identified. The tatA and tatB genes were shown to consitute an operon while tatC is monocistronic. RT-PCR analysis revealed expression of the tat genes during both exponential and stationary growth as well as during intracellular growth in Acanthamoeba castellanii. A search for the conserved twin-arginine motif in predicted signal peptides resulted in a list of putative Tat substrates. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：654 / 661

页数：8

共 46 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].

Altschul, SF ;

Madden, TL ;

Schaffer, AA ;

Zhang, JH ;

Zhang, Z ;

Miller, W ;

Lipman, DJ .

NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402

[2] The Tat protein export pathway [J].

Berks, BC ;

Sargent, F ;

Palmer, T .

MOLECULAR MICROBIOLOGY, 2000, 35 (02) :260-274

[3] The Tat protein translocation pathway and its role in microbial physiology [J].

Berks, BC ;

Palmer, T ;

Sargent, F .

ADVANCES IN MICROBIAL PHYSIOLOGY, VOL 47, 2003, 47 :187-254

[4] A common export pathway for proteins binding complex redox cofactors? [J].

Berks, BC .

MOLECULAR MICROBIOLOGY, 1996, 22 (03) :393-404

[5] Protein transport in the halophilic archaeon ARTICLE Halobacterium sp NRC-1:: a major role for the twin-arginine translocation pathway? [J].

Bolhuis, A .

MICROBIOLOGY-SGM, 2002, 148 :3335-3346

[6] Functional complexity of the twin-arginine translocase TatC component revealed by site-directed mutagenesis [J].

Buchanan, G ;

de Leeuw, E ;

Stanley, NR ;

Wexler, M ;

Berks, BC ;

Sargent, F ;

Palmer, T .

MOLECULAR MICROBIOLOGY, 2002, 43 (06) :1457-1470

[7] Pathogenicity of Legionella pneumophila [J].

Cianciotto, NP .

INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 291 (05) :331-343

[8] Intracellular growth in Acanthamoeba castellanii affects monocyte entry mechanisms and enhances virulence of Legionella pneumophila [J].

Cirillo, JD ;

Cirillo, SLG ;

Yan, L ;

Bermudez, LE ;

Falkow, S ;

Tompkins, LS .

INFECTION AND IMMUNITY, 1999, 67 (09) :4427-4434

[9] Prokaryotic utilization of the twin-arginine translocation pathway:: a genomic survey [J].

Dilks, K ;

Rose, RW ;

Hartmann, E ;

Pohlschröder, M .

JOURNAL OF BACTERIOLOGY, 2003, 185 (04) :1478-1483

[10] Agrobacterium tumefaciens twin-arginine-dependent translocation is important for virulence, flagellation, and chemotaxis but not type IV secretion [J].

Ding, ZY ;

Christie, PJ .

JOURNAL OF BACTERIOLOGY, 2003, 185 (03) :760-771

← 1 2 3 4 5 →