An in vivo study of tricalcium phosphate and glutaraldehyde crosslinking gelatin conduits in peripheral nerve repair

被引:37
作者
Chen, MH
Chen, PR
Chen, MH
Hsieh, ST
Huang, JS
Lin, FH
机构
[1] Natl Taiwan Univ, Inst Biomed Engn, Taipei, Taiwan
[2] Cathay Gen Hosp, Dept Surg, Div Neurosurg, Taipei, Taiwan
[3] Far E Mem Hosp, Dept Internal Med, Taipei, Taiwan
[4] Natl Taiwan Univ, Coll Med, Dept Anat & Cell Biol, Taipei 10764, Taiwan
[5] Natl Taiwan Univ Hosp, Dept Neurol, Taipei, Taiwan
关键词
biodegradable nerve conduit; peripheral nerve regeneration; gelatin; glutaraldehyde; tricalcium phosphate;
D O I
10.1002/jbm.b.30402
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In order to modulate the mechanical properties of gelatin, we previously developed a biodegradable composite composed by tricalcium phosphate and glutaraldehyde crosslinking gelatin (GTG) feasible for surgical manipulation. In this study, we evaluated the in vivo applications of GTG conduit for peripheral nerve repair. The effect of sciatic nerve reconstruction was compared between resorbable permeable GTG conduits and durable impermeable silicone tubes. Traditional methods of assessing nerve recovery following peripheral nerve repair including histomorphometric and electrophysiologic features were conducted in our study. In addition, autotomy score and sciatic function index (SFI) in walking tract analysis were used as additional parameters for assessing the return of nerve function. Twenty-four weeks after sciatic nerve repair, the GTG conduits were harvested. Microscopically, regeneration of nerves was observed in the cross-section at the mid portion of all implanted GTG conduits. The cross-sectional area of regenerated nerve of the GTG group was significant larger than that of the silicone group. In the compound muscle action potentials (CMAP), the mean recovery index of CMAP amplitude was 0.24 +/- 0.02 for the silicone group, 0.41 +/- 0.07 for the GTG group. The mean SFI increased with time in the GTG group during the evaluation period until 24 weeks. Walking tract analysis showed a higher SFI score in the GTG group at both 12 and 24 weeks. The difference reached a significant level at 24 weeks. Thus, the histomorphometric, electrophysiologic, and functional assessments demonstrate that GTG can be a candidate for peripheral nerve repair. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:89 / 97
页数:9
相关论文
共 49 条
[21]   A QUANTITATIVE-EVALUATION OF SUTURE AND TUBULIZATION NERVE REPAIR TECHNIQUES [J].
GIBBY, WA ;
KOERBER, HR ;
HORCH, KW .
JOURNAL OF NEUROSURGERY, 1983, 58 (04) :574-579
[22]   ONGOING ACTIVITY IN SEVERED NERVES - SOURCE AND VARIATION WITH TIME [J].
GOVRINLIPPMANN, R ;
DEVOR, M .
BRAIN RESEARCH, 1978, 159 (02) :406-410
[23]  
GUENARD V, 1992, J NEUROSCI, V12, P3310
[24]   WALKING TRACK ANALYSIS - A LONG-TERM ASSESSMENT OF PERIPHERAL-NERVE RECOVERY [J].
HARE, GMT ;
EVANS, PJ ;
MACKINNON, SE ;
BEST, TJ ;
BAIN, JR ;
SZALAI, JP ;
HUNTER, DA .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1992, 89 (02) :251-258
[25]  
HASTINGS GW, 1984, MACROMOLECULAR BIOMA
[26]   Evaluation of cross-linking procedures of collagen tubes used in peripheral nerve repair [J].
Itoh, S ;
Takakuda, K ;
Kawabata, S ;
Aso, Y ;
Kasai, K ;
Itoh, H ;
Shinomiya, K .
BIOMATERIALS, 2002, 23 (23) :4475-4481
[27]   Sciatic function index, nerve conduction tests, muscle contraction, and axon morphometry as indicators of regeneration [J].
Kanaya, F ;
Firrell, JC ;
Breidenbach, WC .
PLASTIC AND RECONSTRUCTIVE SURGERY, 1996, 98 (07) :1264-1271
[28]   Calcium entry through L-type calcium channels is essential for neurite regeneration in cultured sympathetic neurons [J].
Kulbatski, I ;
Cook, DJ ;
Tator, CH .
JOURNAL OF NEUROTRAUMA, 2004, 21 (03) :357-374
[29]   Biological effects and cytotoxicity of the composite composed by tricalcium phosphate and glutaraldehyde cross-linked gelatin [J].
Lin, FH ;
Yao, CH ;
Sun, JS ;
Liu, HC ;
Huang, CW .
BIOMATERIALS, 1998, 19 (10) :905-917
[30]   TUBULAR REPAIR OF THE MEDIAN NERVE IN THE HUMAN FOREARM - PRELIMINARY FINDINGS [J].
LUNDBORG, G ;
ROSEN, B ;
ABRAHAMSON, SO ;
DAHLIN, L ;
DANIELSEN, N .
JOURNAL OF HAND SURGERY-BRITISH AND EUROPEAN VOLUME, 1994, 19B (03) :273-276