Comparative analysis of ESTs in response to drought stress in chickpea (C. arietinum L.)

被引：43

作者：

Gao, Wen-Rui ^{[2
]}

Wang, Xian-Sheng ^{[2
]}

Liu, Qing-You ^{[1
]}

Peng, Hui ^{[2
]}

Chen, Chen ^{[2
]}

Li, Jian-Gui ^{[3
]}

Zhang, Ju-Song ^{[3
]}

Hu, Song-Nian ^{[1
]}

Ma, Hao ^{[2
]}

机构：

[1] Chinese Acad Sci, Beijing Inst Genom, Beijing 100029, Peoples R China

[2] Nanjing Agr Univ, Dept Agr, Natl Ctr Soybean Improvement, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China

[3] Xinjiang Agr Univ, Key Lab Agr Biotechnol, Urumqi 830052, Xinjiang Uygur, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 376卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Chickpea; Drought stress; Drought tolerance; cDNA library; Expressed sequence tags; Quantitative real-time PCR;

D O I：

10.1016/j.bbrc.2008.09.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chickpea (Cicer arietinum L.) is an important pulse crop grown mainly in the arid and semi-arid regions. To identify the water-stress-induced genes, two non-normalized cDNA libraries were constructed from the seedling leaves of a drought-tolerant chickpea cultivar under PEG-treated and -nontreated conditions. About 2500 clones from each library were selected randomly for sequencing analysis. Based on IDEG6 online software analysis, 92 genes were differentially expressed, and these genes were involved in diverse biological progresses, Such as Metabolism, transcription, signal transduction, protein Synthesis and others. Most of the up-regulated genes were related to drought tolerance, and the down-regulated genes were mainly involved in photosynthesis. The differential expression patterns of five functional unigenes were confirmed by quantitative real-time PCR (qPCR). The results Will help in understanding the molecular basis Of drought tolerance in chickpea. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：578 / 583

页数：6

共 29 条

[1] Plant proteins containing the RNA-recognition motif [J].

Alba, MM ;

Pages, M .

TRENDS IN PLANT SCIENCE, 1998, 3 (01) :15-21

[2] Evaluation of Ethiopian chickpea landraces for tolerance to drought [J].

Anbessa, Y ;

Bejiga, G .

GENETIC RESOURCES AND CROP EVOLUTION, 2002, 49 (06) :557-564

[3] Isolation and characterization of a dehydrin gene from Cicer pinnatifidum, a drought-resistant wild relative of chickpea [J].

Bhattarai, T ;

Fettig, S .

PHYSIOLOGIA PLANTARUM, 2005, 123 (04) :452-458

[4] Strategies for engineering water-stress tolerance in plants [J].

Bohnert, HJ ;

Jensen, RG .

TRENDS IN BIOTECHNOLOGY, 1996, 14 (03) :89-97

[5] Classification of genes differentially expressed during water-deficit stress in Arabidopsis thaliana:: an analysis using Microarray and differential expression data [J].

Bray, EA .

ANNALS OF BOTANY, 2002, 89 :803-811

[6] Plant responses to water deficit [J].

Bray, EA .

TRENDS IN PLANT SCIENCE, 1997, 2 (02) :48-54

[7] Plant responses to drought, from ABA signal transduction events to the action of the induced proteins [J].

Campalans, A ;

Messeguer, R ;

Goday, A ;

Pagès, M .

PLANT PHYSIOLOGY AND BIOCHEMISTRY, 1999, 37 (05) :327-340

[8] ABSCISIC-ACID NEGATIVELY REGULATES EXPRESSION OF CHLOROPHYLL A/B BINDING-PROTEIN GENES DURING SOYBEAN EMBRYOGENY [J].

CHANG, YC ;

WALLING, LL .

PLANT PHYSIOLOGY, 1991, 97 (03) :1260-1264

[9] ISOLATION OF AN OSMOTIC STRESS-INDUCED AND ABSCISIC-ACID-INDUCED GENE ENCODING AN ACIDIC ENDOCHITINASE FROM LYCOPERSICON-CHILENSE [J].

CHEN, RD ;

YU, LX ;

GREER, AF ;

CHERITI, H ;

TABAEIZADEH, Z .

MOLECULAR AND GENERAL GENETICS, 1994, 245 (02) :195-202

[10]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P159

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