Separation and quantification of component monosaccharides of the tea polysaccharides from Gynostemma pentaphyllum by HPLC with indirect UV detection

被引：239

作者：

Lv, You ^{[1
]}

Yang, Xingbin ^{[1
]}

Zhao, Yan ^{[2
]}

Ruan, Yun ^{[1
]}

Yang, Ying ^{[1
]}

Wang, Zhezhi ^{[1
]}

机构：

[1] Shaanxi Normal Univ, Coll Life Sci, Key Lab Minist Educ Med Plant Resource & Nat Phar, Xian 710062, Peoples R China

[2] Fourth Mil Med Univ, Fac Pharmaceut Sci, Xian 710032, Peoples R China

来源：

FOOD CHEMISTRY | 2009年 / 112卷 / 03期

基金：

欧洲研究理事会;

关键词：

Gynostemma pentaphyllum Makino; Tea polysaccharide; Monosaccharide composition; High-performance liquid chromatography; NF-KAPPA-B/REL; LIQUID-CHROMATOGRAPHY; ANTIOXIDANT; ACTIVATION; EXTRACTS; MAKINO;

D O I：

10.1016/j.foodchem.2008.06.042

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A reversed-phase high-performance liquid chromatographic (HPLC) method is described for the simultaneous determination of aldoses and uronic acids. The separation was carried out on a RP-C-18 column (4.6 mm i.d. x 250 mm, 5 pm, Venusil, USA) using precolumn derivatization with 1-phenyl-3-methyl5-pyrazolone (PMP) and UV detection at 250 nm, and the 10 PMP derivatives of mannose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose, arabinose and fucose were baseline separated within 40 min, Furthermore, the described method was applied to the quantitative analysis of component monosaccharides in the water-soluble polysaccharides extracted from Gynostemma pentaphyllum Makino tea and the result showed that the tea polysaccharide was a typical heteropolysaccharide and consisted of marmose, ribose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose and arabinose in the molar contents of 16.3, 10.3, 47.1, 5.6, 24.0, 128.4, 25.0, 101.4 and 71.1 mu M, respectively. Quantitative recoveries of the component monosaccharides in the tea polysaccharide were in the range of 94.6-108.0% and the RSD values were lower than 4.9%. The results demonstrated that the proposed HPLC method was precise and practical for the analysis of the G. pentaphyllum tea polysaccharide. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：742 / 746

页数：5

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