Intestinal reperfusion injury is mediated by IgM and complement

被引:197
作者
Williams, JP
Pechet, TTV
Weiser, MR
Reid, R
Kobzik, L
Moore, FD
Carroll, MC
Hechtman, HB
机构
[1] Brigham & Womens Hosp, Boston, MA 02115 USA
[2] Harvard Univ, Sch Med, Dept Surg, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA
关键词
immunoglobulin M; rodent; inflammation; complement; transgenic/knockout;
D O I
10.1152/jappl.1999.86.3.938
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Intestinal ischemia-reperfusion injury is dependent on complement. This study examines the role of the alternative and classic pathways of complement and IgM in a murine model of intestinal ischemia-reperfusion. Wild-type animals, mice deficient in complement factor 4 (C4), C3, or Ig, or wild-type mice treated with soluble complement receptor 1 were subjected to 40 min of jejunal ischemia and 3 h of reperfusion. Compared with wild types, knockout and treated mice had significantly reduced intestinal injury, indicated by lowered permeability to radiolabeled albumin. When animals deficient in Ig were reconstituted with IgM, the degree of injury was restored to wild-type levels. Immunohistological staining of intestine for C3 and IgM showed colocalization in the mucosa of wild-type controls and minimal staining for both in the intestine of Ig-deficient and C4-deficient mice. We conclude that intestinal ischemia-reperfusion injury is dependent on the classic complement pathway and IgM.
引用
收藏
页码:938 / 942
页数:5
相关论文
共 22 条
  • [1] CHIU CJ, 1970, ARCH SURG-CHICAGO, V101, P478
  • [2] CLEVELAND DW, 1977, J BIOL CHEM, V252, P1102
  • [3] APPEARANCE OF IMMUNOGLOBULIN-G AND COMPLEMENT FACTOR C3 IN THE STRIATUM AFTER TRANSIENT FOCAL ISCHEMIA IN THE RAT
    CZURKO, A
    NISHINO, H
    [J]. NEUROSCIENCE LETTERS, 1994, 166 (01) : 51 - 54
  • [4] Fischer MB, 1996, J IMMUNOL, V157, P549
  • [5] P-selectin mediates intestinal ischemic injury by enhancing complement deposition
    Gibbs, SAL
    Weiser, MR
    Kobzik, L
    Valeri, CR
    Shepro, D
    Hechtman, HB
    [J]. SURGERY, 1996, 119 (06) : 652 - 656
  • [6] ISCHEMIA - REPERFUSION INJURY
    GRACE, PA
    [J]. BRITISH JOURNAL OF SURGERY, 1994, 81 (05) : 637 - 647
  • [7] HILL J, 1992, J IMMUNOL, V149, P1723
  • [8] NITRIC-OXIDE SYNTHASE IN HUMAN AND RAT LUNG - IMMUNOCYTOCHEMICAL AND HISTOCHEMICAL-LOCALIZATION
    KOBZIK, L
    BREDT, DS
    LOWENSTEIN, CJ
    DRAZEN, J
    GASTON, B
    SUGARBAKER, D
    STAMLER, JS
    [J]. AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1993, 9 (04) : 371 - 377
  • [9] IMMUNOGLOBULIN-M ANTIBODIES PRESENT IN THE ACUTE PHASE OF KAWASAKI SYNDROME LYSE CULTURED VASCULAR ENDOTHELIAL-CELLS STIMULATED BY GAMMA INTERFERON
    LEUNG, DYM
    COLLINS, T
    LAPIERRE, LA
    GEHA, RS
    POBER, JS
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1986, 77 (05) : 1428 - 1435
  • [10] RAPID INDUCTION OF NEUTROPHIL ENDOTHELIAL ADHESION BY ENDOTHELIAL COMPLEMENT-FIXATION
    MARKS, RM
    TODD, RF
    WARD, PA
    [J]. NATURE, 1989, 339 (6222) : 314 - 317