Selective amplification of tyv (rfbE), prt (rfbS), viaB, and fliC genes by multiplex PCR for identification of Salmonella enterica

被引：83

作者：

Hirose, K

Itoh, K

Nakajima, H

Kurazono, T

Yamaguchi, M

Moriya, K

Ezaki, T

Kawamura, Y

Tamura, K

Watanabe, H

机构：

[1] Natl Inst Infect Dis, Dept Bacteriol, Shinjuku Ku, Tokyo 1628640, Japan

[2] Natl Publ Hlth Inst, Dept Microbiol, Tokyo 1088638, Japan

[3] Okayama Prefectural Inst Environm Sci & Publ Hlth, Dept Microbiol, Okayama 7010298, Japan

[4] Saitama Inst Publ Hlth, Saitama 3380842, Japan

[5] Saga Prefectural Inst Publ Hlth, Saga 8490925, Japan

[6] Gifu Univ, Sch Med, Dept Microbiol, Gifu 5008750, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 02期

关键词：

D O I：

10.1128/JCM.40.02.633-636.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The PCR primers for O, H, and Vi antigen genes, tyv (rfbE), prt (rfbS),fliC-d, fliC-a, and viaB, were designed and used for the rapid identification of Salmonella enterica serovars Typhi and Paratyphi A with multiplex PCR. The results showed that all the clinical isolates examined of Salmonella serovars Typhi and Paratyphi A were accurately identified by this assay.

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页码：633 / 636

页数：4

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