Population dynamics of polychlorinated biphenyl-dechlorinating microorganisms in contaminated sediments

被引:41
作者
Kim, J
Rhee, GY
机构
[1] SUNY ALBANY,SCH PUBL HLTH,ALBANY,NY 12201
[2] NEW YORK STATE DEPT HLTH,WADSWORTH CTR,ALBANY,NY 12201
关键词
D O I
10.1128/AEM.63.5.1771-1776.1997
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The growth dynamics of polychlorinated biphenyl (PCB)-dechlorinating microorganisms were determined for the first time, along with those of sulfate reducers and methanogens, by using the most-probable-number technique, The time course of Aroclor 1248 dechlorination mirrored the growth of dechlorinators; dechlorination ensued when the dechlorinating population increased by 2 orders of magnitude from 2.5 x 10(5) to 4.6 x 10(7) cells g of sediment(-1), at a specific growth rate of 6.7 day(-1) between 2 and 6 weeks, During this period, PCB-dechlorinating microorganisms dechlorinated Aroclor 1248 at a rate of 3.9 x 10(-8) mol of Cl g of sediment(-1) day(-1), reducing the average number of Cl molecules per biphenyl from 3.9 to 2.8, The growth yield was 4.2 x 10(13) cells mol of Cl dechlorinated(-1). Once dechlorination reached a plateau, after 6 weeks, the number of dechlorinators began to decrease, On the other hand, dechlorinators inoculated into PCB-free sediments decreased over time from their initial level, suggesting that PCBs are required for their selective enrichment, The numbers of sulfate reducers and methanogens increased in both PCB-free and contaminated sediments, showing little difference between them, The maximum population size of sulfate reducers was about an order of magnitude higher than that of dechlorinators, whereas that of methanogens was slightly less, Unlike those of dechlorinators, however, numbers of both sulfate reducers and methanogens remained high even when dechlorination ceased, The results of this study imply that PCB concentrations may have to exceed a certain threshold to maintain the growth of PCB dechlorinators.
引用
收藏
页码:1771 / 1776
页数:6
相关论文
共 34 条
[21]   ESTABLISHMENT OF A POLYCHLORINATED BIPHENYL-DEGRADING ENRICHMENT CULTURE WITH PREDOMINANTLY METADECHLORINATION [J].
MORRIS, PJ ;
MOHN, WW ;
QUENSEN, JF ;
TIEDJE, JM ;
BOYD, SA .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (09) :3088-3094
[22]   EFFECTS OF ORGANIC SUBSTRATES ON DECHLORINATION OF AROCLOR-1242 IN ANAEROBIC SEDIMENTS [J].
NIES, L ;
VOGEL, TM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (09) :2612-2617
[23]   A COMBINED ECOLOGICAL AND PHYSIOLOGICAL APPROACH TO STUDYING SULFATE REDUCTION WITHIN DEEP MARINE SEDIMENT LAYERS [J].
PARKES, RJ ;
CRAGG, BA ;
BALE, SJ ;
GOODMAN, K ;
FRY, JC .
JOURNAL OF MICROBIOLOGICAL METHODS, 1995, 23 (02) :235-249
[24]   FERMENTATION OF INSOLUBLE CELLULOSE BY CONTINUOUS CULTURES OF RUMINOCOCCUS-ALBUS [J].
PAVLOSTATHIS, SG ;
MILLER, TL ;
WOLIN, MJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1988, 54 (11) :2655-2659
[25]   REDUCTIVE DECHLORINATION OF POLYCHLORINATED-BIPHENYLS BY ANAEROBIC MICROORGANISMS FROM SEDIMENTS [J].
QUENSEN, JF ;
TIEDJE, JM ;
BOYD, SA .
SCIENCE, 1988, 242 (4879) :752-754
[26]   DECHLORINATION OF 4 COMMERCIAL POLYCHLORINATED BIPHENYL MIXTURES (AROCLORS) BY ANAEROBIC MICROORGANISMS FROM SEDIMENTS [J].
QUENSEN, JF ;
BOYD, SA ;
TIEDJE, JM .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1990, 56 (08) :2360-2369
[27]  
RHEE GY, 1993, ENVIRON TOXICOL CHEM, V12, P1025, DOI [10.1897/1552-8618(1993)12[1025:RDOAIA]2.0.CO
[28]  
2, 10.1002/etc.5620120608]
[29]   DECHLORINATION OF POLYCHLORINATED-BIPHENYLS BY HUDSON RIVER SEDIMENT ORGANISMS - SPECIFICITY TO THE CHLORINATION PATTERN OF CONGENERS [J].
RHEE, GY ;
SOKOL, RC ;
BETHONEY, CM ;
BUSH, B .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1993, 27 (06) :1190-1192
[30]  
RHEE GY, 1994, GREAT LAKES RES REV, V1, P23