3'-3'-linked oligonucleotides: Synthesis and stability studies

被引：14

作者：

Chaix, C

Iyer, RP

Agrawal, S

机构：

[1] Hybridon Inc., Worcester, MA 01605, One Innovation Drive

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1996年 / 6卷 / 07期

关键词：

D O I：

10.1016/0960-894X(96)00115-1

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

As part of a therapeutic strategy involving multiple targeting, we have synthesized oligonucleotides which are linked via 3'-3' phosphoric diester linkages. These 3'-3' analogs showed increased resistance against nuclease-mediated degradation compared to the 5'-3'-linked oligonucleotides. Copyright (C) 1996 Elsevier Science Ltd

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页码：827 / 832

页数：6

相关论文

共 12 条

[1] PHARMACOKINETICS OF ANTISENSE OLIGONUCLEOTIDES [J].

AGRAWAL, S ;

TEMSAMANI, J ;

GALBRAITH, W ;

TANG, JY .

CLINICAL PHARMACOKINETICS, 1995, 28 (01) :7-16

[2]

Agrawal S., 1994, J BIOTECH HEALTHCARE, V1, P167

[3]

Agrawal Sudhir, 1995, Current Opinion in Biotechnology, V6, P12, DOI 10.1016/0958-1669(95)80003-4

[4]

Beaucage Serge L., 1993, V20, P33

[5] ALTERNATING ALPHA,BETA-OLIGOTHYMIDYLATES WITH ALTERNATING (3'-]3')-INTERNUCLEOTIDIC AND (5'-]5')-INTERNUCLEOTIDIC PHOSPHODIESTER LINKAGES AS MODELS FOR ANTISENSE OLIGODEOXYRIBONUCLEOTIDES [J].

KOGA, M ;

MOORE, MF ;

BEAUCAGE, SL .

JOURNAL OF ORGANIC CHEMISTRY, 1991, 56 (12) :3757-3759

[6]

Ortigao J F, 1992, Antisense Res Dev, V2, P129

[7] ONLINE INTERNAL SURFACE REVERSED-PHASE CLEANING - THE DIRECT HPLC ANALYSIS OF CRUDE BIOLOGICAL SAMPLES - APPLICATION TO THE KINETICS OF DEGRADATION OF OLIGONUCLEOTIDES IN CELL-CULTURE MEDIUM [J].

POMPON, A ;

LEFEBVRE, I ;

IMBACH, JL .

BIOCHEMICAL PHARMACOLOGY, 1992, 43 (08) :1769-1775

[8]

SANDS H, 1994, MOL PHARMACOL, V45, P932

[9]

Temsamani J, 1993, Antisense Res Dev, V3, P277

[10]

UHLMANN E, 1990, CHEM REV, V90, P544