ONLINE INTERNAL SURFACE REVERSED-PHASE CLEANING - THE DIRECT HPLC ANALYSIS OF CRUDE BIOLOGICAL SAMPLES - APPLICATION TO THE KINETICS OF DEGRADATION OF OLIGONUCLEOTIDES IN CELL-CULTURE MEDIUM

被引：33

作者：

POMPON, A

LEFEBVRE, I

IMBACH, JL

机构：

[1] Laboratoire de Chimie Bio-Organique, U.R.A. CNRS No. 488, Université Montpellier II, 34095 Montpellier cedex 5, Case 008, Place E. Bataillon

来源：

BIOCHEMICAL PHARMACOLOGY | 1992年 / 43卷 / 08期

关键词：

D O I：

10.1016/0006-2952(92)90709-R

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

An "on-line" HPLC analysis of crude biological samples is described. A precolumn of internal surface reversed-phase material allows the passage of proteins and other unwanted products while retaining analyses which are transferred, concentrated and chromatographed on a conventional reverse-phase or ion-exchange HPLC column. This protocol allows precise kinetics of the degradation of an oligonucleotide in cell culture to be obtained without radiolabeling or sample preparation,

引用

页码：1769 / 1775

页数：7

共 32 条

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